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Literature summary extracted from

  • Giger, L.; Toscano, M.; Bouzon, M.; Marlière, P.; Hilvert, D.
    A novel genetic selection system for PLP-dependent threonine aldolases (2012), Tetrahedron, 68, 7549-7557.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.26
-
 Caulobacter vibrioides
4.1.2.48
-
 Caulobacter vibrioides

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.48 D176E 500fold decrease in catalytic efficiency Caulobacter vibrioides
4.1.2.48 D95C less than 10% of catalytic efficiency of wild-type Caulobacter vibrioides
4.1.2.48 D95H/E96G less than 10% of catalytic efficiency of wild-type Caulobacter vibrioides
4.1.2.48 D95L less than 10% of catalytic efficiency of wild-type Caulobacter vibrioides
4.1.2.48 D95M less than 5% of catalytic efficiency of wild-type Caulobacter vibrioides
4.1.2.48 D95N/E96S less than 5% of catalytic efficiency of wild-type Caulobacter vibrioides
4.1.2.48 D95W less than 2% of catalytic efficiency of wild-type Caulobacter vibrioides
4.1.2.48 D95Y less than 5% of catalytic efficiency of wild-type Caulobacter vibrioides
4.1.2.48 D95Y/E96T less than 10% of catalytic efficiency of wild-type Caulobacter vibrioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.26 0.035
-
 L-threo-phenylserine wild-type, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.065
-
 L-threo-phenylserine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.071
-
 L-threo-phenylserine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.17
-
 L-threo-phenylserine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.19
-
 L-threo-phenylserine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.24
-
 L-threo-phenylserine mutant D176E, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.31
-
 L-threo-phenylserine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.58
-
 L-threo-phenylserine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.78
-
 L-threo-phenylserine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 2.8
-
 L-threo-phenylserine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.69
-
 L-allo-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 1 2 L-allo-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 1 3 L-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 2.5
-
 L-allo-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 2.7
-
 L-allo-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 2.9
-
 L-allo-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 4.4
-
 L-allo-threonine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 7.1
-
 L-allo-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 11
-
 L-allo-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 13.6
-
 L-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 32
-
 L-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 44
-
 L-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 44
-
 L-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 50
-
 L-threonine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 53
-
 L-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 63
-
 L-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.26 Caulobacter vibrioides Q9A3V8 enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine
-
4.1.2.26 Caulobacter vibrioides CB15 Q9A3V8 enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine
-
4.1.2.48 Caulobacter vibrioides Q9A3V8
-
-
4.1.2.48 Caulobacter vibrioides CB15 Q9A3V8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.26 L-threo-phenylserine
-
 Caulobacter vibrioides glycine + benzaldehyde
-
 ?
4.1.2.26 L-threo-phenylserine
-
 Caulobacter vibrioides CB15 glycine + benzaldehyde
-
 ?
4.1.2.26 additional information enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine Caulobacter vibrioides ?
-
 ?
4.1.2.26 additional information enzyme is a low-specificity L-threonine aldolase, EC 4.1.2.48, and additionally cleaves L-threo-phenylserine Caulobacter vibrioides CB15 ?
-
 ?
4.1.2.48 L-allo-threonine
-
 Caulobacter vibrioides glycine + acetaldehyde
-
 ?
4.1.2.48 L-allo-threonine
-
 Caulobacter vibrioides CB15 glycine + acetaldehyde
-
 ?
4.1.2.48 L-threonine
-
 Caulobacter vibrioides glycine + acetaldehyde
-
 ?
4.1.2.48 L-threonine
-
 Caulobacter vibrioides CB15 glycine + acetaldehyde
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.26 0.1
-
 L-threo-phenylserine mutant D176E, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 0.78
-
 L-threo-phenylserine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 2.8
-
 L-threo-phenylserine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 5.5
-
 L-threo-phenylserine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 6.8
-
 L-threo-phenylserine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 14
-
 L-threo-phenylserine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 21
-
 L-threo-phenylserine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 22
-
 L-threo-phenylserine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 23
-
 L-threo-phenylserine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 67.4
-
 L-threo-phenylserine wild-type, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.2
-
 L-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.2
-
 L-allo-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.6
-
 L-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 3
-
 L-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 3
-
 L-allo-threonine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 3.3
-
 L-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 3.5
-
 L-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 3.6
-
 L-threonine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 3.8
-
 L-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 8.6
-
 L-allo-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 9.6
-
 L-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 19
-
 L-allo-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 19
-
 L-allo-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 20
-
 L-allo-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 20
-
 L-allo-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 22
-
 L-allo-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.26 0.42
-
 L-threo-phenylserine mutant D176E, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 22
-
 L-threo-phenylserine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 36
-
 L-threo-phenylserine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 45
-
 L-threo-phenylserine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 85
-
 L-threo-phenylserine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 96
-
 L-threo-phenylserine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 100
-
 L-threo-phenylserine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 120
-
 L-threo-phenylserine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 130
-
 L-threo-phenylserine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.26 1900
-
 L-threo-phenylserine wild-type, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.0016
-
 L-threonine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.0063
-
 L-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.028
-
 L-allo-threonine mutant D95N/E96S, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.046
-
 L-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.056
-
 L-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.068
-
 L-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.072
-
 L-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.072
-
 L-threonine mutant D95M, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.218
-
 L-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.24
-
 L-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 0.68
-
 L-allo-threonine mutant D95Y/E96T, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 1.7
-
 L-allo-threonine mutant D95C, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 1.8
-
 L-allo-threonine mutant D95L, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 3
-
 L-allo-threonine mutant D95G/E96G, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 7.4
-
 L-allo-threonine mutant D95Y, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 7.6
-
 L-allo-threonine mutant D95W, pH 8.0, 30°C Caulobacter vibrioides
4.1.2.48 29
-
 L-allo-threonine wild-type, pH 8.0, 30°C Caulobacter vibrioides