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Literature summary extracted from
- Characterization of the indole-3-glycerol phosphate synthase from Pseudomonas aeruginosa PAO1 (2012), Protein J., 31, 359-365.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.48 | expressed in Escherichia coli strain KK-8(pDM) | Pseudomonas aeruginosa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.48 | 0.0113 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | at 37°C and pH 8.0 | Pseudomonas aeruginosa |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.48 | 31000 | - |
x * 31000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | Pseudomonas aeruginosa | - |
1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.48 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.48 | TALON metal affinity resin column chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
Pseudomonas aeruginosa | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.48 | ? | x * 31000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.48 | IGPS | - |
Pseudomonas aeruginosa |
| 4.1.1.48 | indole-3-glycerol phosphate synthase | - |
Pseudomonas aeruginosa |
| 4.1.1.48 | TrpC | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.48 | 37 | - |
the enzyme is about 2fold more active at 37°C than it is at 25°C | Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.48 | 11.1 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | at 37°C and pH 8.0 | Pseudomonas aeruginosa | |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.48 | 7 | 8.2 | activity begins to decrease above pH 8.2 and is 70% of maximum at pH 9.0 | Pseudomonas aeruginosa |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.1.1.48 | metabolism | the enzyme catalyzes the fifth reaction in the synthesis of tryptophan from chorismate | Pseudomonas aeruginosa |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.48 | 980 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | at 37°C and pH 8.0 | Pseudomonas aeruginosa | |
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