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Literature summary extracted from

  • Li, C.; Chen, Y.; Zhang, Z.; Gong, C.; Han, H.; Xu, W.
    Glu434 is an important amino acid residue for the activity, structure and stability of tyrosine hydroxylase of the silkworm, Bombyx mori (2013), Process Biochem., 48, 1665-1673.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.2
-
 Bombyx mori

Protein Variants

EC Number Protein Variants Comment Organism
1.14.16.2 E434A the mutant shows 35.6% of wild type activity. Furthermore, the mutation dramatically reduces its substrate affinity for tetrahydrobiopterin and decreases its activation by Fe2+ Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 guanidine hydrochloride no activity at guanidine hydrochloride concentrations exceeding 0.6 M Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.2 0.0263
-
 L-tyrosine recombinant wild type enzyme, at pH 7.5 and 37°C Bombyx mori
1.14.16.2 0.029
-
 L-tyrosine native wild type enzyme, at pH 7.5 and 37°C Bombyx mori
1.14.16.2 0.0428
-
 L-tyrosine mutant enzyme E434A, at pH 7.5 and 37°C Bombyx mori
1.14.16.2 0.0471
-
 tetrahydrobiopterin native wild type enzyme, at pH 7.5 and 37°C Bombyx mori
1.14.16.2 0.0519
-
 tetrahydrobiopterin recombinant wild type enzyme, at pH 7.5 and 37°C Bombyx mori
1.14.16.2 0.341
-
 tetrahydrobiopterin mutant enzyme E434A, at pH 7.5 and 37°C Bombyx mori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.16.2 Fe2+ activates Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.2 66200
-
 x * 66200, SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2 Bombyx mori
-
 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 heparin-Sepharose column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-200HR gel filtration Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 brain
-
 Bombyx mori
-
1.14.16.2 fat body
-
 Bombyx mori
-
1.14.16.2 larva
-
 Bombyx mori
-
1.14.16.2 nerve cord
-
 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + tetrahydrobiopterin + O2
-
 Bombyx mori 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.2 ? x * 66200, SDS-PAGE Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 tyrosine hydroxylase
-
 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.16.2 40 65 minimal aggregation occurs for wild type enzyme after heating at 40°C-45 for 60 min. A steep decrease in activity is observed between 40 and 65°C, and a complete loss of activity occurs at temperatures above 70°C Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.2 tetrahydrobiopterin
-
 Bombyx mori