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Literature summary extracted from

  • Tan, B.K.; Bogdanov, M.; Zhao, J.; Dowhan, W.; Raetz, C.R.; Guan, Z.
    Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates (2012), Proc. Natl. Acad. Sci. USA, 109, 16504-16509.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.B10
-
 Escherichia coli
2.7.8.B11
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.B11 additional information Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Deletion of all three genes (DELTAclsABC) results in a complete lack of cardiolipin. Restoration of near–wild-type levels of cardiolipin production in the stationary phase by expression of clsC in the triple mutant requires the coexpression from the same operon of the preceding gene ymdB. The mode of cardiolipin biosynthesis by the combined YmdB-ClsC follows neither the “eukaryotic” nor “prokaryotic” pathway but involves the transfer of a phosphatidyl moiety from PE to the terminal free hydroxyl of PG to form cardiolipin Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.B10 Escherichia coli P0A6H8 cardiolipin synthase A
-
2.7.8.B10 Escherichia coli P0AA84 cardiolipin synthase B
-
2.7.8.B11 Escherichia coli P75919 cardiolipin synthase C
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.B11 1-(1-heptadecanoyl-2-((9Z)-tetradec-9-enoyl)-sn-glycero-3-phospho)-sn-glycerol + 1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phosphoethanolamine synthetic phosphatidylglycerol (17:0/14:1) and synthetic phosphatidylethanolamine (12:0/13:0). Reaction is catalyzed by the two-component complex YmdB-ClsC. Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Deletion of all three genes (DELTAclsABC) results in a complete lack of cardiolipin. Restoration of near–wild-type levels of cardiolipin production in the stationary phase by expression of clsC in the triple mutant requires the coexpression from the same operon of the preceding gene ymdB. The mode of cardiolipin biosynthesis by the combined YmdB-ClsC follows neither the “eukaryotic” nor “prokaryotic” pathway but involves the transfer of a phosphatidyl moiety from phosphatidylethanolamine to the terminal free hydroxyl of phosphatidylglycerol to form cardiolipin Escherichia coli 1-(1-heptadecanoyl-2-((9Z)-tetradec-9-enoyl)-sn-glycero-3-phospho)-3-(1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phospho)glycerol + ethanolamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.B11 More reaction is catalyzed by the two-component complex YmdB-ClsC Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.8.B10 cardiolipin synthase A
-
 Escherichia coli
2.7.8.B10 cardiolipin synthase B
-
 Escherichia coli
2.7.8.B10 clsA
-
 Escherichia coli
2.7.8.B10 clsB
-
 Escherichia coli
2.7.8.B10 ybhO
-
 Escherichia coli
2.7.8.B11 clsC
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.8.B10 malfunction Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Triple deletions of clsA, clsB, and clsC results in the complete depletion of cardiolipin synthase activity in Escherichia coli cells. The double mutant DELTAclsAB mutant still makes cardiolipin in the stationary phase Escherichia coli
2.7.8.B11 malfunction Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Triple deletions of clsA, clsB, and clsC results in the complete depletion of cardiolipin synthase activity in Escherichia coli cells. The double mutant DELTAclsAB mutant still makes cardiolipin in the stationary phase Escherichia coli
2.7.8.B11 physiological function Escherichia coli has three cardiolipin synthases. All cardiolipin synthases contribute cardiolipin the stationary phase. Only cardiolipin synthase A makes detectible cardiolipin in low-osmolarity medium during logarithmic growth and contributes the majority of cardiolipin in high-osmolarity medium Escherichia coli