Literature summary extracted from

Gwon, H.J.; Yoshioka, H.; Song, N.E.; Kim, J.H.; Song, Y.R.; Jeong, D.Y.; Baik, S.H.
Optimal production of L-threo-2,3-dihydroxyphenylserine (L-threo-DOPS) on a large scale by diastereoselectivity-enhanced variant of L-threonine aldolase expressed in Escherichia coli (2012), Prep. Biochem. Biotechnol., 42, 143-154.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
4.1.2.48	sodium sulfite	addition of sodium sulfite stimulates the production of L-threo-3,4-dihydroxyphenylserine without affecting the diastereoselectivity ratio, especially at 50 mM	Sinorhizobium arboris
4.1.2.48	Triton X-100	highest conversion yield at a 0.75% without affecting the diastereoselectivity ratio	Sinorhizobium arboris

Application

EC Number	Application	Comment	Organism
4.1.2.48	synthesis	production of L-threo-3,4-dihydroxyphenylserine. At the optimized conditions, a mixture of L-threo-3,4-dihydroxyphenylserine and L-erythro-3,4-dihydroxyphenylserine is synthesized by diastereoselectivity-enhanced L-threonine aldolase expressed in Escherichia coli in a continuous process for 100 h, yielding an average of 4.0 mg/ml of L-threo-3,4-dihydroxyphenylserine and 60% diastereoselectivity	Sinorhizobium arboris

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.1.2.48	expression in Escherichia coli	Sinorhizobium arboris

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.2.48	Sinorhizobium arboris	A0T1V9	-	-

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.1.2.48	6.5	-	-	Sinorhizobium arboris

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Release 2023.1 (January 2023)