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Literature summary extracted from

  • Nihira, T.; Saito, Y.; Ohtsubo, K.-i.; , Nakai1, H.; Kitaoka, M.
    2-O-alpha-D-Glucosylglycerol phosphorylase from Bacillus selenitireducens MLS10 possessing hydrolytic activity on beta-D-glucose 1-phosphate (2014), PLoS One, 9, e86548.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.332 expressed in Escherichia coli Salisediminibacterium selenitireducens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.332 E475A inactive mutant Salisediminibacterium selenitireducens
2.4.1.332 E475Q inactive mutant Salisediminibacterium selenitireducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.332 additional information
-
 beta-D-glucose 1-phosphate the Km for beta-D-glucose 1-phosphate in the hydrolytic reaction is 0.041 and 0.16 mM at pH 6.0 and pH 7.5 respectively Salisediminibacterium selenitireducens
2.4.1.332 0.45
-
 beta-D-glucose 1-phosphate pH 7.5, 30°C Salisediminibacterium selenitireducens
2.4.1.332 0.57
-
 phosphate pH 8.0, 30°C Salisediminibacterium selenitireducens
2.4.1.332 1.1
-
 2-O-alpha-D-glucopyranosyl-sn-glycerol pH 8.0, 30°C Salisediminibacterium selenitireducens
2.4.1.332 5.7
-
 sn-glycerol pH 7.5, 30°C Salisediminibacterium selenitireducens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.332 86260
-
 calculated from amino acid sequence Salisediminibacterium selenitireducens
2.4.1.332 87000
-
 2 * 87000, SDS-PAGE Salisediminibacterium selenitireducens
2.4.1.332 174000
-
 gel filtration Salisediminibacterium selenitireducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate Salisediminibacterium selenitireducens
-
 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate Salisediminibacterium selenitireducens DSM 15326
-
 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol Salisediminibacterium selenitireducens
-
 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol Salisediminibacterium selenitireducens DSM 15326
-
 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.332 Salisediminibacterium selenitireducens D6XZ22
-
-
2.4.1.332 Salisediminibacterium selenitireducens DSM 15326 D6XZ22
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.332
-
 Salisediminibacterium selenitireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 Salisediminibacterium selenitireducens beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 Salisediminibacterium selenitireducens DSM 15326 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 ?
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens DSM 15326 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 ?
2.4.1.332 beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens DSM 15326 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
2.4.1.332 additional information 1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose Salisediminibacterium selenitireducens ?
-
 ?
2.4.1.332 additional information 1-butanol, 2-butanol, cyclohexanol, benzyl alcohol, hydroquinone, phenol, ethylene glycol, glycerol, erythritol, xylitol D-arabinitol, L-arabinitol, and D-glucitol are no substitutes for sn-glycerol. With beta-D-glucose 1-phosphate as donor the following compounds are no acceptors: D-glucose, D-mannose, D-allose, D-galactose, D-altrose, D-talose, and D-fructose, D-xylose, D-lyxose, D-ribose, D-arabinose, and L-arabinose, D-glucosamine, D-galactosamine, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine, N-acetyl-D-galactosamine, D-glucuronic acid, D-galacturonic acid, 1,5-anhydro-D-glucitol, 2-deoxy-D-glucose, L-rhamnose, alpha-D-glucose 1-phosphate, D-glucose 6-phosphate, 3-Omethyl-D-glucose, methyl-alpha-D-glucoside, and methyl-beta-D-glucoside, trehalose, kojibiose, nigerose, maltose, isomaltose, sucrose, sophorose, laminaribiose, cellobiose, gentiobiose, lactose, lactulose, melibiose, xylobiose, beta-1,4-mannobiose, N,N'-diacetylchitobiose Salisediminibacterium selenitireducens DSM 15326 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.332 dimer 2 * 87000, SDS-PAGE Salisediminibacterium selenitireducens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.332 2-O-alpha-D-glucopyranosylglycerol phosphorylase
-
 Salisediminibacterium selenitireducens
2.4.1.332 Bsel_2816 locus name Salisediminibacterium selenitireducens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.332 additional information
-
 beta-D-glucose 1-phosphate kcat for beta-D-glucose 1-phosphate in the hydrolytic reaction is 2.8 and 1.7 s(-1) at pH 6.0 and pH 7.5 respectively Salisediminibacterium selenitireducens
2.4.1.332 95
-
 2-O-alpha-D-glucopyranosyl-sn-glycerol pH 8.0, 30°C Salisediminibacterium selenitireducens
2.4.1.332 163
-
 sn-glycerol pH 7.5, 30°C Salisediminibacterium selenitireducens
2.4.1.332 180
-
 beta-D-glucose 1-phosphate pH 7.5, 30°C Salisediminibacterium selenitireducens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.332 additional information
-
 hydrolysis of beta-D-glucose 1-phosphate has an optimum of pH 6.0 Salisediminibacterium selenitireducens
2.4.1.332 7.5
-
 synthesis of 2-O-alpha-D-glucopyranosyl-sn-glycerol Salisediminibacterium selenitireducens
2.4.1.332 8
-
 phosphorolytic activity Salisediminibacterium selenitireducens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.332 5.5 10.5 4°C, 24 h stable Salisediminibacterium selenitireducens