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Literature summary extracted from

  • Jung, W.S.; Singh, R.K.; Lee, J.K.; Pan, C.H.
    Crystal structure and substrate specificity of D-galactose-6-phosphate isomerase complexed with substrates (2013), PLoS ONE, 8, e72902.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.26 expressed in Escherichia coli BL21(DE3) cells Lacticaseibacillus rhamnosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.26 in complex with D-tagatose-6-phosphate, hanging drop vapor diffusion method, using 0.2 M potassium formate containing 20% (w/v) PEG 3350 Lacticaseibacillus rhamnosus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.26 C65A inactive Lacticaseibacillus rhamnosus
5.3.1.26 D8N inactive Lacticaseibacillus rhamnosus
5.3.1.26 H96A the mutant exhibits 25fold lower activity compared with the wild type enzyme activity Lacticaseibacillus rhamnosus
5.3.1.26 H9A inactive Lacticaseibacillus rhamnosus
5.3.1.26 N97A the mutant has an activity level of approximately 30% that of the wild type enzyme Lacticaseibacillus rhamnosus
5.3.1.26 T67A the catalytic activity of the mutant is approximately 20fold lower than that of the wild type enzyme Lacticaseibacillus rhamnosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.26 15000
-
 2 * 19000 + 1 * 15000, SDS-PAGE Lacticaseibacillus rhamnosus
5.3.1.26 19000
-
 2 * 19000 + 1 * 15000, SDS-PAGE Lacticaseibacillus rhamnosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.26 D-Galactose 6-phosphate Lacticaseibacillus rhamnosus
-
 D-Tagatose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.26 Lacticaseibacillus rhamnosus A0A0D6UDD4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.26 HisTrap column chromatography and Superdex 200 gel filtration Lacticaseibacillus rhamnosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.26 D-allose
-
 Lacticaseibacillus rhamnosus D-psicose
-
 ?
5.3.1.26 D-Galactose 6-phosphate
-
 Lacticaseibacillus rhamnosus D-Tagatose 6-phosphate
-
 r
5.3.1.26 D-ribose
-
 Lacticaseibacillus rhamnosus D-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.26 heterotetramer 2 * 19000 + 1 * 15000, SDS-PAGE Lacticaseibacillus rhamnosus

Synonyms

EC Number Synonyms Comment Organism
5.3.1.26 D-galactose-6-phosphate isomerase
-
 Lacticaseibacillus rhamnosus
5.3.1.26 LacAB
-
 Lacticaseibacillus rhamnosus