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Literature summary extracted from

  • Rivas-Pardo, J.A.; Herrera-Morande, A.; Castro-Fernandez, V.; Fernandez, F.J.; Vega, M.C.; Guixe, V.
    Crystal structure, SAXS and kinetic mechanism of hyperthermophilic ADP-dependent glucokinase from Thermococcus litoralis reveal a conserved mechanism for catalysis (2013), PLoS One, 8, e66687.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.147 crystal structures of apo form and holo form, in the presence of D-glucose and the nonhydrolyzable ADP analog adenosine 5'-(3-thio)diphosphate. The conformational changes upon sequential substrate binding can be explained by an almost pure rotation (or a rotation plus a translation) facilitated by residues in the flexible inter-domain connection Thermococcus litoralis
2.7.1.147 sitting-drop vapor-diffusion crystallization Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.147 0.0086
-
 ADP pH not specified in the publication, temperature not specified in the publication Thermococcus litoralis
2.7.1.147 0.0086
-
 ADP 40°C, pH 7.8 Thermococcus litoralis
2.7.1.147 0.218
-
 D-glucose pH not specified in the publication, temperature not specified in the publication Thermococcus litoralis
2.7.1.147 0.219
-
 D-glucose 40°C, pH 7.8 Thermococcus litoralis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Thermococcus litoralis Q7M537
-
-
2.7.1.147 Thermococcus litoralis DSM 5473 Q7M537
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.147
-
 Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.147 ADP + D-glucose
-
 Thermococcus litoralis AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose catalysis follows a sequential ordered mechanism Thermococcus litoralis AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Thermococcus litoralis DSM 5473 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose catalysis follows a sequential ordered mechanism Thermococcus litoralis DSM 5473 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 additional information catalysis follows a sequential ordered mechanism Thermococcus litoralis ?
-
 ?
2.7.1.147 additional information catalysis follows a sequential ordered mechanism Thermococcus litoralis DSM 5473 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.147 ADP-dependent glucokinase
-
 Thermococcus litoralis