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Literature summary extracted from

  • Khare, G.; Gupta, V.; Nangpal, P.; Gupta, R.; Sauter, N.; Tyagi, A.
    Ferritin structure from Mycobacterium tuberculosis: Comparative study with homologues identifies extended C-terminus involved in ferroxidase activity (2011), PLoS ONE, 6, e18570.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.16.3.1 expressed in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.16.3.1 determined at 3 A. The crystallographic data implicate the importance of the extended C-terminal region in the iron entry from the three-fold channels to the ferroxidase centre and making iron more readily accessible for the oxidation Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.16.3.1 additional information a truncated protein (having 1-167 amino acids, molecular weight ,18 kDa) is generated: The truncated protein shows a 3.5fold reduction in the oxidation rate of Fe(II). Lack of C-terminus has an impact of the stability of the protein. Truncated BfrB starts unfolding on exposure to even a very low temperature of 30°C whereas the native protein remains almost unaffected till 50°C before denaturing rapidly Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.16.3.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.16.3.1 using NH4SO4 precipitation and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.3.1 4 Fe2+ + 4 H+ + O2
-
 Mycobacterium tuberculosis 4 Fe3+ + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.16.3.1 bacterioferritin B
-
 Mycobacterium tuberculosis
1.16.3.1 BfrB
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.16.3.1 30
-
 truncated BfrB starts unfolding on exposure to even a very low temperature of 30°C whereas the native protein remains almost unaffected till 50°C before denaturing rapidly Mycobacterium tuberculosis