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Literature summary extracted from

  • Werner, A.K.; Medina-Escobar, N.; Zulawski, M.; Sparkes, I.A.; Cao, F.Q.; Witte, C.P.
    The ureide-degrading reactions of purine ring catabolism employ three amidohydrolases and one aminohydrolase in Arabidopsis, soybean, and rice (2013), Plant Physiol., 163, 672-681.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.116 expressed in Nicotiana benthamiana leaf epidermal cells Glycine max
3.5.1.116 expressed in Nicotiana benthamiana leaf epidermal cells Arabidopsis thaliana
3.5.1.116 expressed in Nicotiana benthamiana leaf epidermal cells Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.116 0.04
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Glycine max
3.5.1.116 0.188
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.116 endoplasmic reticulum predominant localisation Glycine max 5783
-
3.5.1.116 endoplasmic reticulum predominant localisation Arabidopsis thaliana 5783
-
3.5.1.116 endoplasmic reticulum predominant localisation Oryza sativa 5783
-
3.5.1.116 Golgi apparatus
-
 Glycine max 5794
-
3.5.1.116 Golgi apparatus
-
 Arabidopsis thaliana 5794
-
3.5.1.116 Golgi apparatus
-
 Oryza sativa 5794
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.116 (S)-ureidoglycolate + H2O Glycine max
-
 glyoxylate + 2 NH3 + CO2
-
 ?
3.5.1.116 (S)-ureidoglycolate + H2O Arabidopsis thaliana
-
 glyoxylate + 2 NH3 + CO2
-
 ?
3.5.1.116 (S)-ureidoglycolate + H2O Oryza sativa
-
 glyoxylate + 2 NH3 + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.116 Arabidopsis thaliana
-
-
-
3.5.1.116 Glycine max
-
-
-
3.5.1.116 Oryza sativa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.116 leaf
-
 Glycine max
-
3.5.1.116 leaf
-
 Arabidopsis thaliana
-
3.5.1.116 leaf
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.116 20
-
 at pH 7.0 and 37°C Glycine max
3.5.1.116 64
-
 at pH 7.0 and 37°C Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.116 (S)-ureidoglycolate + H2O
-
 Glycine max glyoxylate + 2 NH3 + CO2
-
 ?
3.5.1.116 (S)-ureidoglycolate + H2O
-
 Arabidopsis thaliana glyoxylate + 2 NH3 + CO2
-
 ?
3.5.1.116 (S)-ureidoglycolate + H2O
-
 Oryza sativa glyoxylate + 2 NH3 + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.116 UAH
-
 Glycine max
3.5.1.116 UAH
-
 Arabidopsis thaliana
3.5.1.116 UAH
-
 Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.116 17
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Glycine max
3.5.1.116 55
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.116 295
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Oryza sativa
3.5.1.116 430
-
 (S)-ureidoglycolate at pH 7.0 and 37°C Glycine max