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Literature summary extracted from

  • Fang, Z.P.; Wang, M.; Ruan, Z.R.; Tan, M.; Liu, R.J.; Zhou, M.; Zhou, X.L.; Wang, E.D.
    Coexistence of bacterial leucyl-tRNA synthetases with archaeal tRNA binding domains that distinguish tRNALeu in the archaeal mode (2014), Nucleic Acids Res., 42, 5109-5124.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expressed in Escherichia coli BL21(DE3) cells Natrialba magadii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.4 NaCl no activity of isoform LeuRS1 is detected in NaCl solutions Natrialba magadii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.00335
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.00773
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.00843
-
 L-leucine isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.01101
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.01251
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.01313
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.01643
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.891
-
 L-leucine isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.4 cytoplasm
-
 Natrialba magadii 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.4 K+ the enzyme activity requires high KCl concentration with highest activity in the presence of 3.75 M KCl Natrialba magadii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 102200
-
 x * 102200, isoform LeuRS1, calculated from amino acid sequence Natrialba magadii
6.1.1.4 107600
-
 x * 107600, isoform LeuRS2, calculated from amino acid sequence Natrialba magadii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Natrialba magadii
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu Natrialba magadii ATCC 43099
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Natrialba magadii
-
-
-
6.1.1.4 Natrialba magadii ATCC 43099
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 Ni2+-NTA column chromatography, and gel filtration Natrialba magadii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 AMP + diphosphate + L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
 Natrialba magadii ATP + L-leucine + Pyrococcus horikoshii tRNALeu(GAG)
-
 r
6.1.1.4 AMP + diphosphate + L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
 Natrialba magadii ATCC 43099 ATP + L-leucine + Pyrococcus horikoshii tRNALeu(GAG)
-
 r
6.1.1.4 ATP + L-leucine + Natrialba magadii tRNALeu(CAA)
-
 Natrialba magadii AMP + diphosphate + L-leucyl-Natrialba magadii tRNALeu(CAA)
-
 ?
6.1.1.4 ATP + L-leucine + Natrialba magadii tRNALeu(CAA)
-
 Natrialba magadii ATCC 43099 AMP + diphosphate + L-leucyl-Natrialba magadii tRNALeu(CAA)
-
 ?
6.1.1.4 ATP + L-leucine + Natrialba magadii tRNALeu(GAG) highest activity Natrialba magadii AMP + diphosphate + L-leucyl-Natrialba magadii tRNALeu(GAG)
-
 ?
6.1.1.4 ATP + L-leucine + Natrialba magadii tRNALeu(GAG) highest activity Natrialba magadii ATCC 43099 AMP + diphosphate + L-leucyl-Natrialba magadii tRNALeu(GAG)
-
 ?
6.1.1.4 ATP + L-leucine + Pyrococcus horikoshii tRNALeu(GAG) 100% activity Natrialba magadii AMP + diphosphate + L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
 r
6.1.1.4 ATP + L-leucine + Pyrococcus horikoshii tRNALeu(GAG) 100% activity Natrialba magadii ATCC 43099 AMP + diphosphate + L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
 r
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Natrialba magadii AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Natrialba magadii ATCC 43099 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 ? x * 102200, isoform LeuRS1, calculated from amino acid sequence Natrialba magadii
6.1.1.4 ? x * 107600, isoform LeuRS2, calculated from amino acid sequence Natrialba magadii

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucyl-tRNA synthetase
-
 Natrialba magadii
6.1.1.4 LeuRS1 isoform, exhibits much higher aminoacylation and editing activity than isoform LeuRS2 Natrialba magadii
6.1.1.4 LeuRS2 isoform, exhibits much less aminoacylation and editing activity than isoform LeuRS1 Natrialba magadii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 0.000418
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.000668
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.00119
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.00961
-
 L-leucine isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.0418
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.0464
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.064
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.563
-
 L-leucine isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 9
-
 in Tris-HCl or Bis-Tris-propane-HCl, the activity decreases obviously at pH 8.0 or below Natrialba magadii
6.1.1.4 9.5
-
 in CHES-KOH, the activity decreases obviously at pH 8.0 or below Natrialba magadii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
 Natrialba magadii

General Information

EC Number General Information Comment Organism
6.1.1.4 metabolism isoform LeuRS1 generates Leu-tRNALeu for protein biosynthesis and exhibits obvious post-transfer editing activity to prevent generation of mischarged tRNALeu Natrialba magadii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.4 0.0108
-
 L-leucine isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.0318
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.0724
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 0.199
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 3.34
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 4.21
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 9.28
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
6.1.1.4 66.8
-
 L-leucine isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii