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Literature summary extracted from

  • Ahmed, W.; Bhat, A.G.; Leelaram, M.N.; Menon, S.; Nagaraja, V.
    Carboxyl terminal domain basic amino acids of mycobacterial topoisomerase I bind DNA to promote strand passage (2013), Nucleic Acids Res., 41, 7462-7471.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.1 expression of the tagged enzyme in Escherichia coli strain BL21(DE3) from plasmid pPVN123, cloningi in Escherichia coli strain DH10B Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.1 additional information generation of deletion mutants of the enzyme lacking each one of the three basic stretches of the C-terminal domain. The mutants are highly compromised in binding two DNA molecules Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.1 Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.2.1 additional information Mycolicibacterium smegmatis MstopoI carries out the DNA relaxation reaction in a processive fashion ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.1 recombinant tagged enzyme from Escherichia coli strain BL21(DE3) by heparin affinity chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.1 additional information MstopoI carries out the DNA relaxation reaction in a processive fashion Mycolicibacterium smegmatis ?
-
 ?
5.6.2.1 additional information DNA cleavage assay using a 32-mer oligonucleotide harboring strong topoI sites to yield a 5'-end-labeled 19-mer cleavage product. For intramolecular religation, 32-mer oligonucleotide harboring strong topoI sites is incubated with wild-type or deletants to yield a noncovalenty held 19-mer and 13-mer covalent protein-DNA adduct Mycolicibacterium smegmatis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.1 topoI
-
 Mycolicibacterium smegmatis
5.6.2.1 Topoisomerase I
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.1 37
-
 assay at Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.1 8
-
 assay at Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
5.6.2.1 additional information the C-terminal domain contains three basic stretches that are essential for the DNA relaxation activity. The basic amino acid stretches confer the non-specific DNA-binding property to the C-terminal domain, overview Mycolicibacterium smegmatis