Any feedback?
Literature summary extracted from
- CYP4V2 in Biettis crystalline dystrophy: ocular localization, metabolism of omega-3-polyunsaturated fatty acids, and functional deficit of the p.H331P variant (2012), Mol. Pharmacol., 82, 679-686.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.79 | expression in insect cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.79 | H331P | the naturally occuring mutant protein CYP4V2 protein is expressed at high levels in ocular target tissues of patients with Biettis crystalline corneoretinal dystrophy. The enzyme is metabolically active toward polyunsaturated fatty acids. The functional deficit among patients with Biettis crystalline corneoretinal dystrophy who carry the H331P variant is most likely a consequence of the instability of the mutant protein | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.79 | endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| 1.14.14.79 | microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.79 | 60000 | - |
x * 60000, SDS-PAGE | Homo sapiens |
| 1.14.14.79 | 62163 | - |
x * 62163, liquid chromatography-mass spectrometry analysis | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.79 | docosahexaenoate + NADPH + H+ + O2 | Homo sapiens | - |
22-hydroxydocosahexaenoate + NADP+ + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.79 | Homo sapiens | Q6ZWL3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.79 | isolated through nickel affinity and hydroxyapatite chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.79 | adrenal gland | - |
Homo sapiens | - |
| 1.14.14.79 | ARPE-19 cell | a human cell line spontaneously generated from normal human retinal pigmented epithelium | Homo sapiens | - |
| 1.14.14.79 | breast | - |
Homo sapiens | - |
| 1.14.14.79 | cornea | - |
Homo sapiens | - |
| 1.14.14.79 | eye | - |
Homo sapiens | - |
| 1.14.14.79 | hepatocyte | - |
Homo sapiens | - |
| 1.14.14.79 | hypophysis | endothelial cell | Homo sapiens | - |
| 1.14.14.79 | liver | - |
Homo sapiens | - |
| 1.14.14.79 | additional information | negligible amount is found in human kidney. No expression in small intestine | Homo sapiens | - |
| 1.14.14.79 | pancreas | islet cells | Homo sapiens | - |
| 1.14.14.79 | parathyroid gland | - |
Homo sapiens | - |
| 1.14.14.79 | prostate gland | - |
Homo sapiens | - |
| 1.14.14.79 | retina | - |
Homo sapiens | - |
| 1.14.14.79 | testis | - |
Homo sapiens | - |
| 1.14.14.79 | uterus | glandular epithelial cells | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.79 | (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoic acid + [reduced NADPH-hemoprotein reductase] + O2 | icosapentaenoate i.e. (5Z,8Z,11Z,14Z,17Z)-icosa-5,8,11,14,17-pentaenoate | Homo sapiens | (5Z,8Z,11Z,14Z,17Z)-20-hydroxyicosa-5,8,11,14,17-pentaenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.79 | docosahexaenoate + NADPH + H+ + O2 | - |
Homo sapiens | 22-hydroxydocosahexaenoate + NADP+ + H2O | - |
? | |
| 1.14.14.79 | docosahexaenoate + NADPH + H+ + O2 | docosahexaenoate i.e. (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoate. The enzyme also produces some 21-hydroxydocosahexaenoate | Homo sapiens | 22-hydroxydocosahexaenoate + NADP+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.79 | ? | x * 60000, SDS-PAGE | Homo sapiens |
| 1.14.14.79 | ? | x * 62163, liquid chromatography-mass spectrometry analysis | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.79 | CYP4F3B | - |
Homo sapiens |
| 1.14.14.79 | CYP4V2 | - |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.79 | cytochrome P-450 | CYP4V2 displays a typical reduced carbon monoxide-bound P450 spectrum | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.79 | malfunction | defects in the enzyme are associated with Bietti crystalline corneoretinal dystrophy | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
