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Literature summary extracted from

  • Schlame, M.; Zhao, M.; Rua, D.; Haldar, D.; Greenberg, M.L.
    Kinetic analysis of cardiolipin synthase: a membrane enzyme with two glycerophospholipid substrates (1995), Lipids, 30, 633-640.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.41 additional information
-
 additional information the two Km values for CDP-diacylglycerol, each estimated by nonlinear regression analysis of 18 kinetic points, are 0.02 and 0.03 mol% in the presence of 1 mM and 10 mM Mg2+ respectively, pH 9.0, 37°C Saccharomyces cerevisiae
2.7.8.41 0.0024
-
 CDP-1,2-dimyristoylglycerol at low Co2+ concentration, pH pH 9.0, 37°C Rattus norvegicus
2.7.8.41 0.0029
-
 CDP-1,2-dimyristoylglycerol at high Co2+ concentration, pH pH 9.0, 37°C Rattus norvegicus
2.7.8.41 0.032
-
 egg yolk phosphatidylglycerol at high Co2+ concentration, pH pH 9.0, 37°C Rattus norvegicus
2.7.8.41 0.0619
-
 egg yolk phosphatidylglycerol at low Co2+ concentration, pH pH 9.0, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.41 mitochondrion
-
 Rattus norvegicus 5739
-
2.7.8.41 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.41 Co2+ most efficient cation for the activation. Co2+ activates cardiolipin formation by increasing the maximal enzyme activity from 2.9 pmol/min/mg protein at low Co2+ concentration to 4.1 pmol/min/mg protein at high Co2+ concentration Rattus norvegicus
2.7.8.41 Mg2+ 1.7 m required for half-maximal activity Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.41 a CDP-diacylglycerol + a phosphatidyl-glycerol Rattus norvegicus
-
 a cardiolipin + CMP
-
 ?
2.7.8.41 a CDP-diacylglycerol + a phosphatidyl-glycerol Saccharomyces cerevisiae
-
 a cardiolipin + CMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.41 Rattus norvegicus
-
-
-
2.7.8.41 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.41 partial Rattus norvegicus
2.7.8.41 partial Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.41 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.41 0.0000029
-
 at low Co2+ concentration, pH 9.0, 37°C Rattus norvegicus
2.7.8.41 0.0000041
-
 at high Co2+ concentration, pH 9.0, 37°C Rattus norvegicus
2.7.8.41 0.000513
-
 pH 9.0, 37°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.41 a CDP-diacylglycerol + a phosphatidyl-glycerol
-
 Rattus norvegicus a cardiolipin + CMP
-
 ?
2.7.8.41 a CDP-diacylglycerol + a phosphatidyl-glycerol
-
 Saccharomyces cerevisiae a cardiolipin + CMP
-
 ?
2.7.8.41 a CDP-diacylglycerol + a phosphatidylglycerol
-
 Saccharomyces cerevisiae a cardiolipin + CMP
-
 ?
2.7.8.41 CDP-1,2-dimyristoylglycerol + egg yolk phosphatidylglycerol
-
 Rattus norvegicus a cardiolipin + CMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.41 37
-
 assay at Rattus norvegicus
2.7.8.41 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.41 9
-
 assay at Rattus norvegicus
2.7.8.41 9
-
 assay at Saccharomyces cerevisiae