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Literature summary extracted from

  • Welin, M.; Lehtiö, L.; Johansson, A.; Flodin, S.; Nyman, T.; Trésaugues, L.; Hammarström, M.; Gräslund, S.; Nordlund, P.
    Substrate specificity and oligomerization of human GMP synthetase (2013), J. Mol. Biol., 425, 4323-4333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.2 expressed in Escherichia coli BL21(DE3) gold pRARE cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.5.2 in complex with XMP, sitting drop vapor diffusion method, using 0.1 M sodium acetate trihydrate, pH 5.2, 1.6 M ammonium sulfate, and 0.2 M sodium chloride, at 25Ā°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.2 70000
-
 2 * 70000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.2 ATP + XMP + L-glutamine + H2O Homo sapiens the N-terminal glutaminase domain of the enzyme generates ammonia from glutamine and the C-terminal synthetase domain aminates xanthine monophosphate to form GMP AMP + diphosphate + GMP + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.2 Homo sapiens P49915
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.2 Ni-charged HiTrap chelating column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.2 ATP + XMP + L-glutamine + H2O the N-terminal glutaminase domain of the enzyme generates ammonia from glutamine and the C-terminal synthetase domain aminates xanthine monophosphate to form GMP Homo sapiens AMP + diphosphate + GMP + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.2 homodimer 2 * 70000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.3.5.2 GMP synthetase
-
 Homo sapiens
6.3.5.2 GMPS
-
 Homo sapiens
6.3.5.2 Guanine monophosphate synthetase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.2 ATP
-
 Homo sapiens