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Literature summary extracted from

  • Lee, H.J.; Kwon, H.W.; Koh, J.U.; Lee, D.K.; Moon, J.Y.; Kong, K.H.
    An efficient method for the expression and reconstitution of thermostable Mn/Fe superoxide dismutase from Aeropyrum pernix K1 (2011), J. Microbiol. Biotechnol., 20, 727-731.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 overexpressed as a GST fusion protein at a high level in Escherichia coli Aeropyrum pernix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ the enzyme is active with either Fe(II) or Mn(II) as a cofactor. The recombinant enzyme is produced in Escherichia coli expressed as an apoprotein. This apoprotein shows no SOD activity. The recombinant is activated with Fe(NH4)2(SO4)2 and MnSO4 salts at elevated temperature. The Fe-reconstituted enzyme contains 0.79 atom of iron per subunit Aeropyrum pernix
1.15.1.1 Mn2+ the enzyme is active with either Fe(II) or Mn(II) as a cofactor. The recombinant enzyme is produced in Escherichia coli expressed as an apoprotein. This apoprotein shows no SOD activity. The recombinant is activated with Fe(NH4)2(SO4)2 and MnSO4 salts at elevated temperature. The Mn-reconstituted enzyme contains 0.82 atom of manganese per subunit Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 25000
-
 2 * 25000, SDS-PAGE Aeropyrum pernix
1.15.1.1 56000
-
 gel filtration Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Aeropyrum pernix Q9YE27
-
-
1.15.1.1 Aeropyrum pernix DSM 11879 Q9YE27
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1
-
 Aeropyrum pernix

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 25000, SDS-PAGE Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 APE0743
-
 Aeropyrum pernix