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Literature summary extracted from

  • Klinkenberg, L.G.; Karakousis, P.C.
    Rv1894c is a novel hypoxia-induced nitronate monooxygenase required for Mycobacterium tuberculosis virulence (2013), J. Infect. Dis., 207, 1525-1534.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.16 expressed as a His-tagged fusion protein in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.16 H199A mutant shows no enzymatic function Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.16 Superoxide dismutase
-
 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.16 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.16 using Ni-NTA chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.16 2-nitropropane + O2
-
 Mycobacterium tuberculosis acetone + HNO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.16 nitronate monooxygenase
-
 Mycobacterium tuberculosis
1.13.12.16 Rv1894c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.16 37
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.16 FMN
-
 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
1.13.12.16 Mycobacterium tuberculosis negatively regulated by the transcriptional repressor KstR down