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Thiol synthetases of legumes: immunogold localization and differential gene regulation by phytohormones

Clemente, M.R.; Bustos-Sanmamed, P.; Loscos, J.; James, E.K.; Perez-Rontome, C.; Navascues, J.; Gay, M.; Becana, M.; J. Exp. Bot. 63, 3923-3934 (2012)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.2.23
auxin
activates enzyme activity
Lotus japonicus
6.3.2.23
auxin
activates enzyme activity
Medicago sativa
6.3.2.23
auxin
activates enzyme activity
Phaseolus vulgaris
6.3.2.23
additional information
no activation by cyctokinins and polyamines
Lotus japonicus
6.3.2.23
additional information
no activation by cyctokinins and polyamines
Medicago sativa
6.3.2.23
additional information
no activation by cyctokinins and polyamines
Phaseolus vulgaris
Cloned(Commentary)
EC Number
Commentary
Organism
6.3.2.23
recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Lotus japonicus
6.3.2.23
recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Medicago sativa
6.3.2.23
recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Phaseolus vulgaris
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.3.2.23
chloroplast
-
Lotus japonicus
9507
-
6.3.2.23
chloroplast
-
Medicago sativa
9507
-
6.3.2.23
chloroplast
-
Phaseolus vulgaris
9507
-
6.3.2.23
cytosol
-
Lotus japonicus
5829
-
6.3.2.23
cytosol
-
Medicago sativa
5829
-
6.3.2.23
cytosol
-
Phaseolus vulgaris
5829
-
6.3.2.23
additional information
immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview
Lotus japonicus
-
-
6.3.2.23
additional information
immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview
Medicago sativa
-
-
6.3.2.23
plastid
-
Lotus japonicus
9536
-
6.3.2.23
plastid
-
Medicago sativa
9536
-
6.3.2.23
plastid
-
Phaseolus vulgaris
9536
-
6.3.2.23
starch grain
of leaf chloroplasts and root plastids
Medicago sativa
43036
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.2.23
Mg2+
required
Lotus japonicus
6.3.2.23
Mg2+
required
Medicago sativa
6.3.2.23
Mg2+
required
Phaseolus vulgaris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.2.23
50000
-
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Lotus japonicus
6.3.2.23
50000
-
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Medicago sativa
6.3.2.23
50000
-
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Phaseolus vulgaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
Phaseolus vulgaris
-
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
?
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
Medicago sativa
-
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
?
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
Lotus japonicus
-
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.2.23
Lotus japonicus
-
ecotype MG-20, in nodulated plants x Mesorhizobium loti R7A
-
6.3.2.23
Medicago sativa
-
cv. Aragon x Sinorhizobium meliloti 102F78
-
6.3.2.23
Phaseolus vulgaris
-
cv. Contender x Rhizobium leguminosarum cv. phaseoli 3622
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.2.23
recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography
Lotus japonicus
6.3.2.23
recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography
Medicago sativa
6.3.2.23
recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography
Phaseolus vulgaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.2.23
leaf
-
Lotus japonicus
-
6.3.2.23
leaf
mainly in starch grains within chloroplasts
Medicago sativa
-
6.3.2.23
additional information
immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview
Phaseolus vulgaris
-
6.3.2.23
root
nonnodulated and nodulated plants
Lotus japonicus
-
6.3.2.23
root
nodulated plants, mainly in starch grains within plastids
Medicago sativa
-
6.3.2.23
root
nodulated plants
Phaseolus vulgaris
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
728056
Phaseolus vulgaris
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
-
?
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
728056
Medicago sativa
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
-
?
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
728056
Lotus japonicus
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.3.2.23
?
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Lotus japonicus
6.3.2.23
?
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Medicago sativa
6.3.2.23
?
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Phaseolus vulgaris
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.3.2.23
30
-
assay at
Lotus japonicus
6.3.2.23
30
-
assay at
Medicago sativa
6.3.2.23
30
-
assay at
Phaseolus vulgaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.23
8.5
-
assay at
Lotus japonicus
6.3.2.23
8.5
-
assay at
Medicago sativa
6.3.2.23
8.5
-
assay at
Phaseolus vulgaris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.3.2.23
ATP
-
Lotus japonicus
6.3.2.23
ATP
-
Medicago sativa
6.3.2.23
ATP
-
Phaseolus vulgaris
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.3.2.23
auxin
activates enzyme activity
Lotus japonicus
6.3.2.23
auxin
activates enzyme activity
Medicago sativa
6.3.2.23
auxin
activates enzyme activity
Phaseolus vulgaris
6.3.2.23
additional information
no activation by cyctokinins and polyamines
Lotus japonicus
6.3.2.23
additional information
no activation by cyctokinins and polyamines
Medicago sativa
6.3.2.23
additional information
no activation by cyctokinins and polyamines
Phaseolus vulgaris
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.23
recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Lotus japonicus
6.3.2.23
recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Medicago sativa
6.3.2.23
recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Phaseolus vulgaris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.3.2.23
ATP
-
Lotus japonicus
6.3.2.23
ATP
-
Medicago sativa
6.3.2.23
ATP
-
Phaseolus vulgaris
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.3.2.23
chloroplast
-
Lotus japonicus
9507
-
6.3.2.23
chloroplast
-
Medicago sativa
9507
-
6.3.2.23
chloroplast
-
Phaseolus vulgaris
9507
-
6.3.2.23
cytosol
-
Lotus japonicus
5829
-
6.3.2.23
cytosol
-
Medicago sativa
5829
-
6.3.2.23
cytosol
-
Phaseolus vulgaris
5829
-
6.3.2.23
additional information
immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview
Lotus japonicus
-
-
6.3.2.23
additional information
immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview
Medicago sativa
-
-
6.3.2.23
plastid
-
Lotus japonicus
9536
-
6.3.2.23
plastid
-
Medicago sativa
9536
-
6.3.2.23
plastid
-
Phaseolus vulgaris
9536
-
6.3.2.23
starch grain
of leaf chloroplasts and root plastids
Medicago sativa
43036
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.2.23
Mg2+
required
Lotus japonicus
6.3.2.23
Mg2+
required
Medicago sativa
6.3.2.23
Mg2+
required
Phaseolus vulgaris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.2.23
50000
-
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Lotus japonicus
6.3.2.23
50000
-
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Medicago sativa
6.3.2.23
50000
-
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Phaseolus vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
Phaseolus vulgaris
-
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
?
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
Medicago sativa
-
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
?
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
Lotus japonicus
-
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.2.23
recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography
Lotus japonicus
6.3.2.23
recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography
Medicago sativa
6.3.2.23
recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography
Phaseolus vulgaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.2.23
leaf
-
Lotus japonicus
-
6.3.2.23
leaf
mainly in starch grains within chloroplasts
Medicago sativa
-
6.3.2.23
additional information
immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview
Phaseolus vulgaris
-
6.3.2.23
root
nonnodulated and nodulated plants
Lotus japonicus
-
6.3.2.23
root
nodulated plants, mainly in starch grains within plastids
Medicago sativa
-
6.3.2.23
root
nodulated plants
Phaseolus vulgaris
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
728056
Phaseolus vulgaris
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
-
?
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
728056
Medicago sativa
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
-
?
6.3.2.23
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
728056
Lotus japonicus
ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
i.e. homo-glutathione
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.2.23
?
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Lotus japonicus
6.3.2.23
?
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Medicago sativa
6.3.2.23
?
x * 50000, about, recombinant tagged enzyme, SDS-PAGE
Phaseolus vulgaris
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
6.3.2.23
30
-
assay at
Lotus japonicus
6.3.2.23
30
-
assay at
Medicago sativa
6.3.2.23
30
-
assay at
Phaseolus vulgaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.2.23
8.5
-
assay at
Lotus japonicus
6.3.2.23
8.5
-
assay at
Medicago sativa
6.3.2.23
8.5
-
assay at
Phaseolus vulgaris
Expression
EC Number
Organism
Commentary
Expression
6.3.2.23
Lotus japonicus
abscisic acid downregulates the enzyme in nonnodulated plants, salicylic acid downregulates the enzyme slightly in nodulated plants, jasmonic acid, and auxin downregulate the enzyme in roots, cytokinins and polyamines reduce the enzyme slightly in nodulated plants
down
6.3.2.23
Lotus japonicus
abscisic acid upregulates the enzyme slightly in nodulated plants, salicylic acid upregulates the enzyme slightly in nonnodulated plants, indole-3-acetic acid induces the enzyme slightly in nodulated plants
up
General Information
EC Number
General Information
Commentary
Organism
6.3.2.23
metabolism
the response profile of GSH and hGSH in nonnodulated and nodulated plants to treatment with various hormones indicate that thiol homeostasis is independent of the nitrogen source of the plants
Lotus japonicus
6.3.2.23
physiological function
differential regulation of mRNA levels, enzyme activity, and thiol contents by hormones, overview. Glutathione and homoglutathione play distinct roles in plant development and stress response
Lotus japonicus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
6.3.2.23
metabolism
the response profile of GSH and hGSH in nonnodulated and nodulated plants to treatment with various hormones indicate that thiol homeostasis is independent of the nitrogen source of the plants
Lotus japonicus
6.3.2.23
physiological function
differential regulation of mRNA levels, enzyme activity, and thiol contents by hormones, overview. Glutathione and homoglutathione play distinct roles in plant development and stress response
Lotus japonicus
Expression (protein specific)
EC Number
Organism
Commentary
Expression
6.3.2.23
Lotus japonicus
abscisic acid downregulates the enzyme in nonnodulated plants, salicylic acid downregulates the enzyme slightly in nodulated plants, jasmonic acid, and auxin downregulate the enzyme in roots, cytokinins and polyamines reduce the enzyme slightly in nodulated plants
down
6.3.2.23
Lotus japonicus
abscisic acid upregulates the enzyme slightly in nodulated plants, salicylic acid upregulates the enzyme slightly in nonnodulated plants, indole-3-acetic acid induces the enzyme slightly in nodulated plants
up