Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Clemente, M.R.; Bustos-Sanmamed, P.; Loscos, J.; James, E.K.; Perez-Rontome, C.; Navascues, J.; Gay, M.; Becana, M.
    Thiol synthetases of legumes: immunogold localization and differential gene regulation by phytohormones (2012), J. Exp. Bot., 63, 3923-3934.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.23 auxin activates enzyme activity Phaseolus vulgaris
6.3.2.23 auxin activates enzyme activity Medicago sativa
6.3.2.23 auxin activates enzyme activity Lotus japonicus
6.3.2.23 additional information no activation by cyctokinins and polyamines Phaseolus vulgaris
6.3.2.23 additional information no activation by cyctokinins and polyamines Medicago sativa
6.3.2.23 additional information no activation by cyctokinins and polyamines Lotus japonicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.23 recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Phaseolus vulgaris
6.3.2.23 recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Medicago sativa
6.3.2.23 recombinant expression of N-terminally maltose binding protein- and His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Lotus japonicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.23 chloroplast
-
 Phaseolus vulgaris 9507
-
6.3.2.23 chloroplast
-
 Medicago sativa 9507
-
6.3.2.23 chloroplast
-
 Lotus japonicus 9507
-
6.3.2.23 cytosol
-
 Phaseolus vulgaris 5829
-
6.3.2.23 cytosol
-
 Medicago sativa 5829
-
6.3.2.23 cytosol
-
 Lotus japonicus 5829
-
6.3.2.23 additional information immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview Medicago sativa
-
-
6.3.2.23 additional information immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview Lotus japonicus
-
-
6.3.2.23 plastid
-
 Phaseolus vulgaris 9536
-
6.3.2.23 plastid
-
 Medicago sativa 9536
-
6.3.2.23 plastid
-
 Lotus japonicus 9536
-
6.3.2.23 starch grain of leaf chloroplasts and root plastids Medicago sativa 43036
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.23 Mg2+ required Phaseolus vulgaris
6.3.2.23 Mg2+ required Medicago sativa
6.3.2.23 Mg2+ required Lotus japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.23 50000
-
 x * 50000, about, recombinant tagged enzyme, SDS-PAGE Phaseolus vulgaris
6.3.2.23 50000
-
 x * 50000, about, recombinant tagged enzyme, SDS-PAGE Medicago sativa
6.3.2.23 50000
-
 x * 50000, about, recombinant tagged enzyme, SDS-PAGE Lotus japonicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine Phaseolus vulgaris
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homo-glutathione ?
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine Medicago sativa
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homo-glutathione ?
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine Lotus japonicus
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homo-glutathione ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.23 Lotus japonicus
-
 ecotype MG-20, in nodulated plants x Mesorhizobium loti R7A
-
6.3.2.23 Medicago sativa
-
 cv. Aragon x Sinorhizobium meliloti 102F78
-
6.3.2.23 Phaseolus vulgaris
-
 cv. Contender x Rhizobium leguminosarum cv. phaseoli 3622
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.23 recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography Phaseolus vulgaris
6.3.2.23 recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography Medicago sativa
6.3.2.23 recombinant N-terminally maltose binding protein- and His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and immuno affinity chromatography Lotus japonicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.23 leaf
-
 Lotus japonicus
-
6.3.2.23 leaf mainly in starch grains within chloroplasts Medicago sativa
-
6.3.2.23 additional information immunolocalization studies of thiol synthetases in leaves, roots, and nodules, overview Phaseolus vulgaris
-
6.3.2.23 root nodulated plants Phaseolus vulgaris
-
6.3.2.23 root nodulated plants, mainly in starch grains within plastids Medicago sativa
-
6.3.2.23 root nonnodulated and nodulated plants Lotus japonicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Phaseolus vulgaris ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homo-glutathione ?
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Medicago sativa ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homo-glutathione ?
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine
-
 Lotus japonicus ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine i.e. homo-glutathione ?

Subunits

EC Number Subunits Comment Organism
6.3.2.23 ? x * 50000, about, recombinant tagged enzyme, SDS-PAGE Phaseolus vulgaris
6.3.2.23 ? x * 50000, about, recombinant tagged enzyme, SDS-PAGE Medicago sativa
6.3.2.23 ? x * 50000, about, recombinant tagged enzyme, SDS-PAGE Lotus japonicus

Synonyms

EC Number Synonyms Comment Organism
6.3.2.23 hGSHS
-
 Phaseolus vulgaris
6.3.2.23 hGSHS
-
 Medicago sativa
6.3.2.23 hGSHS
-
 Lotus japonicus
6.3.2.23 homoglutathione synthetase
-
 Phaseolus vulgaris
6.3.2.23 homoglutathione synthetase
-
 Medicago sativa
6.3.2.23 homoglutathione synthetase
-
 Lotus japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.23 30
-
 assay at Phaseolus vulgaris
6.3.2.23 30
-
 assay at Medicago sativa
6.3.2.23 30
-
 assay at Lotus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.23 8.5
-
 assay at Phaseolus vulgaris
6.3.2.23 8.5
-
 assay at Medicago sativa
6.3.2.23 8.5
-
 assay at Lotus japonicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.23 ATP
-
 Phaseolus vulgaris
6.3.2.23 ATP
-
 Medicago sativa
6.3.2.23 ATP
-
 Lotus japonicus

Expression

EC Number Organism Comment Expression
6.3.2.23 Lotus japonicus abscisic acid downregulates the enzyme in nonnodulated plants, salicylic acid downregulates the enzyme slightly in nodulated plants, jasmonic acid, and auxin downregulate the enzyme in roots, cytokinins and polyamines reduce the enzyme slightly in nodulated plants down
6.3.2.23 Lotus japonicus abscisic acid upregulates the enzyme slightly in nodulated plants, salicylic acid upregulates the enzyme slightly in nonnodulated plants, indole-3-acetic acid induces the enzyme slightly in nodulated plants up

General Information

EC Number General Information Comment Organism
6.3.2.23 metabolism the response profile of GSH and hGSH in nonnodulated and nodulated plants to treatment with various hormones indicate that thiol homeostasis is independent of the nitrogen source of the plants Lotus japonicus
6.3.2.23 physiological function differential regulation of mRNA levels, enzyme activity, and thiol contents by hormones, overview. Glutathione and homoglutathione play distinct roles in plant development and stress response Lotus japonicus