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Literature summary extracted from

  • Brisson, L.; El Bakkali-Taheri, N.; Giorgi, M.; Fadel, A.; Kaizer, J.; Reglier, M.; Tron, T.; Ajandouz, e.l..H.; Simaan, A.J.
    1-Aminocyclopropane-1-carboxylic acid oxidase: insight into cofactor binding from experimental and theoretical studies (2012), J. Biol. Inorg. Chem., 17, 939-949.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.17.4 bicarbonate the enzyme requires the presence of CO2 or bicarbonate for activity Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.17.4 expressed in Escherichia coli BL21(DE3) cells Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.17.4 (1-amino-1-methyl)ethylphosphonic acid competitive inhibitor versus both 1-aminocyclopropane-1-carboxylate and bicarbonate Solanum lycopersicum
1.14.17.4 1-aminocyclopropane-1-phosphonic acid competitive inhibitor versus both 1-aminocyclopropane-1-carboxylate and bicarbonate Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.17.4 0.15
-
 1-aminocyclopropane-1-carboxylate in 50 mM MOPS, pH 7.0, at 29°C Solanum lycopersicum
1.14.17.4 2.7
-
 ascorbate in 50 mM MOPS, pH 7.0, at 29°C Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.17.4 Fe2+ a nonheme Fe(II)-containing enzyme Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 + H+ Solanum lycopersicum
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.17.4 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.17.4 DEAE Sepharose resin column chromatography Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.17.4 1-aminocyclopropane-1-carboxylate + ascorbate + O2 + H+
-
 Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.17.4 1-aminocyclopropane-1-carboxylic acid oxidase
-
 Solanum lycopersicum
1.14.17.4 ACC oxidase
-
 Solanum lycopersicum
1.14.17.4 ACCO
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.17.4 0.19
-
 1-aminocyclopropane-1-carboxylate in 50 mM MOPS, pH 7.0, at 29°C Solanum lycopersicum
1.14.17.4 0.2
-
 ascorbate in 50 mM MOPS, pH 7.0, at 29°C Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.17.4 ascorbate
-
 Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.17.4 1.5
-
 1-aminocyclopropane-1-phosphonic acid in 50 mM MOPS, pH 7.0, at 29°C Solanum lycopersicum
1.14.17.4 2.3
-
 (1-amino-1-methyl)ethylphosphonic acid in 50 mM MOPS, pH 7.0, at 29°C Solanum lycopersicum