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Literature summary extracted from

  • Velez, N.; Brautigam, C.A.; Phillips, M.A.
    Trypanosoma brucei S-adenosylmethionine decarboxylase N terminus is essential for allosteric activation by the regulatory subunit prozyme (2013), J. Biol. Chem., 288, 5232-5240.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.50 additional information enzyme activation likely involves a conformational change of the N-terminal peptide Trypanosoma brucei
4.1.1.50 putrescine
-
 Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.50 expressed in Escherichia coli Top10 cells Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.50 0.14
-
 S-adenosyl-L-methionine wild type homodimeric enzyme, with 5 mM putrescine, at pH 8.0 and 37°C Trypanosoma brucei
4.1.1.50 0.4
-
 S-adenosyl-L-methionine wild type homodimeric enzyme, without putrescine, at pH 8.0 and 37°C Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine Trypanosoma brucei
-
 S-adenosyl 3-(methylthio)propylamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.50 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.50 Ni2+-agarose column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine
-
 Trypanosoma brucei S-adenosyl 3-(methylthio)propylamine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.50 homodimer
-
 Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
4.1.1.50 AdoMetDC
-
 Trypanosoma brucei
4.1.1.50 S-Adenosylmethionine decarboxylase
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.50 0.0058
-
 S-adenosyl-L-methionine wild type homodimeric enzyme, without putrescine, at pH 8.0 and 37°C Trypanosoma brucei
4.1.1.50 0.0065
-
 S-adenosyl-L-methionine wild type homodimeric enzyme, with 5 mM putrescine, at pH 8.0 and 37°C Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.50 15000
-
 S-adenosyl-L-methionine wild type homodimeric enzyme, without putrescine, at pH 8.0 and 37°C Trypanosoma brucei
4.1.1.50 46000
-
 S-adenosyl-L-methionine wild type homodimeric enzyme, with 5 mM putrescine, at pH 8.0 and 37°C Trypanosoma brucei