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Literature summary extracted from

  • Filisetti, D.; Theobald-Dietrich, A.; Mahmoudi, N.; Rudinger-Thirion, J.; Candolfi, E.; Frugier, M.
    Aminoacylation of Plasmodium falciparum tRNAAsn and insights in the synthesis of asparagine repeats (2013), J. Biol. Chem., 288, 36361-36371.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.22 expressed in Escherichia coli Top10 cells Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.22 0.002
-
tRNAAsn in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
6.1.1.22 0.04
-
L-asparagine in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
6.1.1.22 0.74
-
ATP in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.22 cytosol
-
Plasmodium falciparum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.22 127000
-
2 * 127000, calculated from amino acid sequence Plasmodium falciparum
6.1.1.22 295000
-
dynamic light scattering Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.22 ATP + L-asparagine + tRNAAsn Plasmodium falciparum
-
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.22 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.22 Ni-NTA resin column chromatography Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
Plasmodium falciparum AMP + diphosphate + L-asparaginyl-tRNAAsn
-
?

Subunits

EC Number Subunits Comment Organism
6.1.1.22 homodimer 2 * 127000, calculated from amino acid sequence Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
6.1.1.22 AsnRS
-
Plasmodium falciparum
6.1.1.22 Asparaginyl-tRNA synthetase
-
Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.22 0.5
-
tRNAAsn in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
6.1.1.22 4.2
-
L-asparagine in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
6.1.1.22 10.1
-
ATP in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.22 ATP
-
Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.22 4.2
-
tRNAAsn in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
6.1.1.22 13.6
-
ATP in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum
6.1.1.22 105
-
L-asparagine in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C Plasmodium falciparum