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Literature summary extracted from
- Carotenoid isomerase is key determinant of petal color of Calendula officinalis (2012), J. Biol. Chem., 287, 276-285.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.2.1.13 | gene CoCRTISO1-ORa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
| 5.2.1.13 | gene CoCRTISO1-ORb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
| 5.2.1.13 | gene CoCRTISO1-Y, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
| 5.2.1.13 | gene CoCRTISO2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
| 5.2.1.13 | gene CoCRTISO3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
| 5.2.1.13 | gene CoCRTISO4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR analysis | Calendula officinalis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.2.1.13 | A412S | site-directed mutagenesis, the mutant shows substrate specificity like the wild-type enzyme | Calendula officinalis |
| 5.2.1.13 | G450E | site-directed mutagenesis, the isozyme CoCRTISO1-ORb mutant is catalytically inactive | Calendula officinalis |
| 5.2.1.13 | I370T | site-directed mutagenesis, the isozyme CoCRTISO1-ORa mutant is catalytically inactive | Calendula officinalis |
| 5.2.1.13 | L581P | site-directed mutagenesis, the isozyme CoCRTISO1-ORa mutant is catalytically inactive | Calendula officinalis |
| 5.2.1.13 | P584L | site-directed mutagenesis, the mutant shows substrate specificity like the wild-type enzyme | Calendula officinalis |
| 5.2.1.13 | S412A | site-directed mutagenesis, the isozyme CoCRTISO1-ORb mutant is catalytically inactive | Calendula officinalis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.2.1.13 | additional information | Calendula officinalis | isozyme substrate specificity in different genotypes, overview | ? | - |
? |  |
| 5.2.1.13 | additional information | Calendula officinalis | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color | ? | - |
? | |
| 5.2.1.13 | additional information | Calendula officinalis | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color | ? | - |
? | |
| 5.2.1.13 | additional information | Calendula officinalis | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.2.1.13 | Calendula officinalis | G9MAL3 | isozyme CoCRTISO1-ORa; gene crtiso | - |
| 5.2.1.13 | Calendula officinalis | G9MAL4 | isozyme CoCRTISO1-ORb; gene crtiso | - |
| 5.2.1.13 | Calendula officinalis | G9MAL5 | isozyme CoCRTISO1-Y; gene crtiso | - |
| 5.2.1.13 | Calendula officinalis | G9MAL6 | isozyme CoCRTISO2; gene crtiso | - |
| 5.2.1.13 | Calendula officinalis | G9MAL7 | isozyme CoCRTISO3; gene crtiso | - |
| 5.2.1.13 | Calendula officinalis | G9MAL8 | isozyme CoCRTISO4; gene crtiso | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.2.1.13 | leaf | - |
Calendula officinalis | - |
| 5.2.1.13 | petal | - |
Calendula officinalis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.2.1.13 | (5'Z)-gamma-carotene | - |
Calendula officinalis | (all-E)-gamma-carotene | - |
? | |
| 5.2.1.13 | (5'Z)-gamma-carotene | no activity with the wild-type, but with mutant 1-ORa462insCHH | Calendula officinalis | (all-E)-gamma-carotene | - |
? | |
| 5.2.1.13 | (5'Z)-gamma-carotene | no activity with the wild-type, but with variant E450G | Calendula officinalis | (all-E)-gamma-carotene | - |
? | |
| 5.2.1.13 | (5'Z)-gamma-carotene | wild-type and mutants I370T and A412S | Calendula officinalis | (all-E)-gamma-carotene | - |
? | |
| 5.2.1.13 | (5'Z)-rubixanthin | no activity with the wild-type, but with mutant 1-ORa462insCHH | Calendula officinalis | (all-E)-rubixanthin | - |
? | |
| 5.2.1.13 | (5'Z)-rubixanthin | no activity with the wild-type, but with variant E450G | Calendula officinalis | (all-E)-rubixanthin | - |
? | |
| 5.2.1.13 | (5'Z)-rubixanthin | wild-type and mutants I370T and A412S | Calendula officinalis | (all-E)-rubixanthin | - |
? | |
| 5.2.1.13 | (5Z,9Z,5'Z,9'Z)-lycopene | - |
Calendula officinalis | (all-E)-lycopene | - |
? | |
| 5.2.1.13 | (5Z,9Z,5'Z,9'Z)-lycopene | no activity with the wild-type, but with mutant 1-ORa462insCHH | Calendula officinalis | (all-E)-lycopene | - |
? | |
| 5.2.1.13 | (5Z,9Z,5'Z,9'Z)-lycopene | no activity with the wild-type, but with variant E450G | Calendula officinalis | (all-E)-lycopene | - |
? | |
| 5.2.1.13 | (5Z,9Z,5'Z,9'Z)-lycopene | wild-type and mutants I370T and A412S | Calendula officinalis | (all-E)-lycopene | - |
? | |
| 5.2.1.13 | additional information | isozyme substrate specificity in different genotypes, overview | Calendula officinalis | ? | - |
? | |
| 5.2.1.13 | additional information | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORa, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color | Calendula officinalis | ? | - |
? | |
| 5.2.1.13 | additional information | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-ORb, which is expressed in orange petals, shows no activity with any of the cis-carotenoids tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines link closely to petal color | Calendula officinalis | ? | - |
? | |
| 5.2.1.13 | additional information | isozyme substrate specificity in different genotypes, overview. Isozyme CoCRTISO1-Y, which is expressed in yellow petals, converts carotenoids from the cis-to-trans-configuration | Calendula officinalis | ? | - |
? | |
| 5.2.1.13 | prolycopene | - |
Calendula officinalis | (all-E)-lycopene | - |
? | |
| 5.2.1.13 | prolycopene | no activity with the wild-type, but with mutant 1-ORa462insCHH | Calendula officinalis | (all-E)-lycopene | - |
? | |
| 5.2.1.13 | prolycopene | no activity with the wild-type, but with variant E450G | Calendula officinalis | (all-E)-lycopene | - |
? | |
| 5.2.1.13 | prolycopene | wild-type and mutants I370T and A412S | Calendula officinalis | (all-E)-lycopene | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.2.1.13 | carotenoid isomerase | - |
Calendula officinalis |
| 5.2.1.13 | CoCRTISO1-ORa | - |
Calendula officinalis |
| 5.2.1.13 | CoCRTISO1-ORb | - |
Calendula officinalis |
| 5.2.1.13 | CoCRTISO1Y | - |
Calendula officinalis |
| 5.2.1.13 | CoCRTISO2 | - |
Calendula officinalis |
| 5.2.1.13 | CoCRTISO3 | - |
Calendula officinalis |
| 5.2.1.13 | CoCRTISO4 | - |
Calendula officinalis |
| 5.2.1.13 | CRTISO | - |
Calendula officinalis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.2.1.13 | malfunction | the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids | Calendula officinalis |
| 5.2.1.13 | malfunction | the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. A Gly-to-Glu amino acid substitution at position 450 in CoCRTISO1-ORb abolishes enzyme activity completely | Calendula officinalis |
| 5.2.1.13 | malfunction | the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. Deletion of Cys-His-His at positions 462-464 in isozyme CoCRTISO1-ORa abolishes enzyme activity completely | Calendula officinalis |
| 5.2.1.13 | additional information | residue Gly450 in isozyme CoCRTISO1-ORb is important for the enzymatic activity of the CRTISO isozyme | Calendula officinalis |
| 5.2.1.13 | additional information | residues Cys-His-His at positions 462-464 in isozymes CoCRTISO1-ORa are important for the enzymatic activity of the CRTISO isozyme | Calendula officinalis |
| 5.2.1.13 | physiological function | isozyme CoCRTISO1 is a key regulator of the accumulation of 5-cis-carotenoids in calendula petals | Calendula officinalis |
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