Literature summary extracted from
Nakamura, A.; Fujihashi, M.; Aono, R.; Sato, T.; Nishiba, Y.; Yoshida, S.; Yano, A.; Atomi, H.; Imanaka, T.; Miki, K.
Dynamic, ligand-dependent conformational change triggers reaction of ribose-1,5-bisphosphate isomerase from Thermococcus kodakarensis KOD1 (2012), J. Biol. Chem., 287, 20784-20796.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.3.1.29 |
expression in Escherichia coli |
Thermococcus kodakarensis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.3.1.29 |
sitting drop vapor diffusion method at 20°C. Crystal structure of unliganded Tk-R15Pi is solved by means of the single-wavelength anomalous dispersion method using SeMet-substituted enzyme and refined at 2.5 A resolution |
Thermococcus kodakarensis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.3.1.29 |
C133A |
inactive mutant enzyme |
Thermococcus kodakarensis |
5.3.1.29 |
C133S |
inactive mutant enzyme |
Thermococcus kodakarensis |
5.3.1.29 |
D202N |
inactive mutant enzyme |
Thermococcus kodakarensis |
5.3.1.29 |
R227R |
mutant enzyme exhibits a decrease in molecular size of the enzyme and significantly decreases the enzymatic activity |
Thermococcus kodakarensis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.3.1.29 |
Thermococcus kodakarensis |
Q5JFM9 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.3.1.29 |
- |
Thermococcus kodakarensis |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
5.3.1.29 |
29.3 |
- |
pH 8.0, 85°C, wild-type enzyme |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.3.1.29 |
alpha-D-ribose 1,5-bisphosphate |
the enzyme is specific for the alpha-anomer of D-ribose 1,5-bisphosphate |
Thermococcus kodakarensis |
D-ribulose 1,5-bisphosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.3.1.29 |
hexamer |
- |
Thermococcus kodakarensis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.3.1.29 |
R15Pi |
- |
Thermococcus kodakarensis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
5.3.1.29 |
85 |
- |
assay at |
Thermococcus kodakarensis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.3.1.29 |
8 |
- |
assay at |
Thermococcus kodakarensis |