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Literature summary extracted from

  • Acharya, P.; Goenrich, M.; Hagemeier, C.H.; Demmer, U.; Vorholt, J.A.; Thauer, R.K.; Ermler, U.
    How an enzyme binds the C1 carrier tetrahydromethanopterin. Structure of the tetrahydromethanopterin-dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1 (2005), J. Biol. Chem., 280, 13712-13719.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.147 expressed in Escherichia coli BL21(DE3) pLysS cells Methylorubrum extorquens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.147 non-labeled and selenomethionine-labeled enzyme, hanging drop vapor diffusion method, using 0.2 M calcium chloride hydrate, 0.1 M sodium acetate hydrate, pH 4.6, and 10-20% (v/v) isopropanol Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde Methylorubrum extorquens
-
 5,10-methylenetetrahydromethanopterin + H2O
-
 ?
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
 5,10-methylenetetrahydromethanopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.147 Methylorubrum extorquens Q9FA38
-
-
4.2.1.147 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 Q9FA38
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.147
-
 Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde
-
 Methylorubrum extorquens 5,10-methylenetetrahydromethanopterin + H2O
-
 ?
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 5,10-methylenetetrahydromethanopterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.147 homopentamer x-ray crystallography Methylorubrum extorquens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.147 FAE
-
 Methylorubrum extorquens
4.2.1.147 formaldehyde-activating enzyme
-
 Methylorubrum extorquens

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.147 tetrahydromethanopterin dependent on Methylorubrum extorquens

General Information

EC Number General Information Comment Organism
4.2.1.147 physiological function formaldehyde-activating enzyme is essential for energy metabolism and formaldehyde detoxification Methylorubrum extorquens