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Literature summary extracted from

  • Baumer, S.; Ide, T.; Jacobi, C.; Johann, A.; Gottschalk, G.; Deppenmeier, U.
    The F420H2 dehydrogenase from Methanosarcina mazei is a Redox-driven proton pump closely related to NADH dehydrogenases (2000), J. Biol. Chem., 275, 17968-17973.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.98.3 expression in Escherichia coli Methanosarcina mazei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.98.3 cytoplasmic membrane
-
 Methanosarcina mazei
-
-
1.12.98.3 membrane membrane-bound enzyme Methanosarcina mazei 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.3 115000
-
 gel filtration Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.98.3 reduced coenzyme F420 + methanophenazine Methanosarcina mazei
-
 oxidized coenzyme F420 + dihydromethanophenazine
-
 ?
1.5.98.3 reduced coenzyme F420 + methanophenazine Methanosarcina mazei Goe1
-
 oxidized coenzyme F420 + dihydromethanophenazine
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine Methanosarcina mazei coenzyme F420-dependent reduction of 2-hydroxyphenazine as catalyzed by the membrane-bound enzyme is coupled to proton translocation across the cytoplasmic membrane, exhibiting a stoichiometry of 0.9 H+ translocated per two electrons transferred. The electrochemical proton gradient thereby generated drives ATP synthesis from ADP and diphosphate 2-dihydropentaprenyloxyphenazine
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine Methanosarcina mazei DSM 3647 coenzyme F420-dependent reduction of 2-hydroxyphenazine as catalyzed by the membrane-bound enzyme is coupled to proton translocation across the cytoplasmic membrane, exhibiting a stoichiometry of 0.9 H+ translocated per two electrons transferred. The electrochemical proton gradient thereby generated drives ATP synthesis from ADP and diphosphate 2-dihydropentaprenyloxyphenazine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.3 Methanosarcina mazei
-
-
-
1.5.98.3 Methanosarcina mazei Goe1
-
-
-
1.12.98.3 Methanosarcina mazei Q8PU58 and F1SVL1 and Q8PZ67 and F1SVH7 and F1SVE4 and Q8PU59 and Q8PU60 and Q8PU61 and F1SVE0 and F1SVK0 and F1SVH9 and F1SVL2 and F1SVE1 Q8PU58: subunit A, F1SVL1: subunit C, Q8PZ67: subunit F, F1SVH7: subunit B, F1SVE4: subunit D, Q8PU59: subunit H, Q8PU60: subunit I, Q8PU61: subunit J, F1SVE0: subunit K, F1SVK0; subunit L, F1SVH9: subunit M, F1SVL2: subunit N, F1SVE1: subunit O
-
1.12.98.3 Methanosarcina mazei DSM 3647 Q8PU58 and F1SVL1 and Q8PZ67 and F1SVH7 and F1SVE4 and Q8PU59 and Q8PU60 and Q8PU61 and F1SVE0 and F1SVK0 and F1SVH9 and F1SVL2 and F1SVE1 Q8PU58: subunit A, F1SVL1: subunit C, Q8PZ67: subunit F, F1SVH7: subunit B, F1SVE4: subunit D, Q8PU59: subunit H, Q8PU60: subunit I, Q8PU61: subunit J, F1SVE0: subunit K, F1SVK0; subunit L, F1SVH9: subunit M, F1SVL2: subunit N, F1SVE1: subunit O
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.3 reduced coenzyme F420 + methanophenazine
-
 Methanosarcina mazei oxidized coenzyme F420 + dihydromethanophenazine
-
 ?
1.5.98.3 reduced coenzyme F420 + methanophenazine
-
 Methanosarcina mazei Goe1 oxidized coenzyme F420 + dihydromethanophenazine
-
 ?
1.5.98.3 reduced coenzyme F420 + oxidized 2-hydroxyphenazine
-
 Methanosarcina mazei oxidized coenzyme F420 + reduced 2-hydroxyphenazine
-
 ?
1.5.98.3 reduced coenzyme F420 + oxidized 2-hydroxyphenazine
-
 Methanosarcina mazei Goe1 oxidized coenzyme F420 + reduced 2-hydroxyphenazine
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine coenzyme F420-dependent reduction of 2-hydroxyphenazine as catalyzed by the membrane-bound enzyme is coupled to proton translocation across the cytoplasmic membrane, exhibiting a stoichiometry of 0.9 H+ translocated per two electrons transferred. The electrochemical proton gradient thereby generated drives ATP synthesis from ADP and diphosphate Methanosarcina mazei 2-dihydropentaprenyloxyphenazine
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine cofactor F420(H2)-dependent reduction of 2-hydroxyphenazine as catalyzed by the membrane-bound enzyme is coupled to proton translocation across the cytoplasmic membrane, exhibiting a stoichiometry of 0.9 H+ translocated per two electrons transferred Methanosarcina mazei 2-dihydropentaprenyloxyphenazine
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine coenzyme F420-dependent reduction of 2-hydroxyphenazine as catalyzed by the membrane-bound enzyme is coupled to proton translocation across the cytoplasmic membrane, exhibiting a stoichiometry of 0.9 H+ translocated per two electrons transferred. The electrochemical proton gradient thereby generated drives ATP synthesis from ADP and diphosphate Methanosarcina mazei DSM 3647 2-dihydropentaprenyloxyphenazine
-
 ?
1.12.98.3 H2 + 2-(2,3-dihydropentaprenyloxy)phenazine cofactor F420(H2)-dependent reduction of 2-hydroxyphenazine as catalyzed by the membrane-bound enzyme is coupled to proton translocation across the cytoplasmic membrane, exhibiting a stoichiometry of 0.9 H+ translocated per two electrons transferred Methanosarcina mazei DSM 3647 2-dihydropentaprenyloxyphenazine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.98.3 F420H2 dehydrogenase
-
 Methanosarcina mazei
1.5.98.3 FpoAHJKLMN
-
 Methanosarcina mazei

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.3 FAD
-
 Methanosarcina mazei
1.5.98.3 iron-sulfur center the enzyme contains iron-sulfur clusters Methanosarcina mazei
1.12.98.3 coenzyme F420 the reaction is dependent on the cofactor F420 Methanosarcina mazei

General Information

EC Number General Information Comment Organism
1.12.98.3 physiological function the enzyme is part of the energy conserving electron transport system of the methanogenic archaeon Methanosarcina mazei Gö1 Methanosarcina mazei