Any feedback?
Literature summary extracted from
- Effect of cold shock on lipid A biosynthesis in Escherichia coli. Induction At 12 degrees C of an acyltransferase specific for palmitoleoyl-acyl carrier protein (1999), J. Biol. Chem., 274, 9677-9685.
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.242 | a palmitoleoyl-[acyl-carrier protein] + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | Escherichia coli | the enzyme is involved in the formation of a cold-adapted lipid A. It transfers palmitoleate from palmitoleoyl-[acyl-carrier protein] to Kdo2-lipid IVA, which is also the acceptor for the lpxL-encoded lauroyl transferase. Palmitoleate is not present in lipid A isolated from Escherichia coli grown at 30°C or higher, but it comprises about 11% of the fatty acyl chains of lipid A in cells grown at 12°C. The appearance of palmitoleate at 12°C is accompanied by a decline in laurate from 18% to 5.5%. The palmitoleoyl transferase is induced more than 30fold upon cold shock. The replacement of laurate with palmitoleate in lipid A may reflect the desirability of maintaining the optimal outer membrane fluidity at 12°C | (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-((9Z)-hexadec-9-enoyloxy)tetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + an [acyl-carrier protein] | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.1.242 | Escherichia coli | P0ACV2 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.242 | a palmitoleoyl-[acyl-carrier protein] + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | the enzyme is involved in the formation of a cold-adapted lipid A. It transfers palmitoleate from palmitoleoyl-[acyl-carrier protein] to Kdo2-lipid IVA, which is also the acceptor for the lpxL-encoded lauroyl transferase. Palmitoleate is not present in lipid A isolated from Escherichia coli grown at 30°C or higher, but it comprises about 11% of the fatty acyl chains of lipid A in cells grown at 12°C. The appearance of palmitoleate at 12°C is accompanied by a decline in laurate from 18% to 5.5%. The palmitoleoyl transferase is induced more than 30fold upon cold shock. The replacement of laurate with palmitoleate in lipid A may reflect the desirability of maintaining the optimal outer membrane fluidity at 12°C | Escherichia coli | (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-((9Z)-hexadec-9-enoyloxy)tetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + an [acyl-carrier protein] | - |
? | |
| 2.3.1.242 | a palmitoleoyl-[acyl-carrier protein] + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose | the enzyme is highly selective for palmitoleoyl-[acyl-carrier protein]. Lauroyl-[acyl-carrier protein], myristoyl--[acyl-carrier protein], palmitoyl--[acyl-carrier protein], R-3-hydroxymyristoyl--[acyl-carrier protein], palmitoleoyl-coenzyme A, and palmitoylcoenzyme A are virtually inactive as substrates | Escherichia coli | (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-((9Z)-hexadec-9-enoyloxy)tetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + an [acyl-carrier protein] | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.1.242 | cold-induced palmitoleoyl transferase | - |
Escherichia coli |
| 2.3.1.242 | LpxP | - |
Escherichia coli |
| 2.3.1.242 | palmitoleoyl-acyl carrier protein-dependent acyltransferase | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.242 | 12 | - |
assay at | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.242 | 7.5 | - |
assay at | Escherichia coli |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 2.3.1.242 | Escherichia coli | the elevated specific activity of the palmitoleoyl transferase under conditions of cold shock is attributed to greatly increased levels of lpxP mRNA. Induced more than 30fold upon cold shock, as judged by assaying extracts of cells shifted to 12°C. The induced activity is maximal after 2 h of cold shock, and then gradually declines but does not disappear | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.3.1.242 | physiological function | the enzyme is involved in the formation of a cold-adapted lipid A. It transfers palmitoleate from palmitoleoyl-[acyl-carrier protein] to Kdo2-lipid IVA, which is also the acceptor for the lpxL-encoded lauroyl transferase. Palmitoleate is not present in lipid A isolated from Escherichia coli grown at 30°C or higher, but it comprises about 11% of the fatty acyl chains of lipid A in cells grown at 12 °C. The appearance of palmitoleate at 12°C is accompanied by a decline in laurate from 18% to 5.5%. The palmitoleoyl transferase is induced more than 30fold upon cold shock. The replacement of laurate with palmitoleate in lipid A may reflect the desirability of maintaining the optimal outer membrane fluidity at 12°C | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
