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Literature summary extracted from

  • Saiki, K.; Mogi, T.; Ogura, K.; Anraku, Y.
    In vitro heme O synthesis by the cyoE gene product from Escherichia coli (1993), J. Biol. Chem., 268, 26041-26044.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.141 Dithionite presence of reducing agents is essential, and dithionite is most effective among reagents tested Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.141 cytoplasmic membrane
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.141 Ca2+ the CyoE-overproduced membranes efficiently catalyze a conversion of exogenous ferrous protohemMe and farnesyl diphosphate to heme i n the presence of divalent cations such as Mg2+ or Ca2+ Escherichia coli
2.5.1.141 Mg2+ the CyoE-overproduced membranes efficiently catalyze a conversion of exogenous ferrous protohemMe and farnesyl diphosphate to heme i n the presence of divalent cations such as Mg2+ or Ca2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.141 26000
-
 x * 26000, SDS-PAGE Escherichia coli
2.5.1.141 28000
-
 x * 28000, urea-SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.141 protoheme IX + (2E,6E)-farnesyl diphosphate + H2O Escherichia coli
-
 heme o + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.141 Escherichia coli P0AEA5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.141 protoheme IX + (2E,6E)-farnesyl diphosphate + H2O
-
 Escherichia coli heme o + diphosphate
-
 ?
2.5.1.141 protoheme IX + (2E,6E)-farnesyl diphosphate + H2O the CyoE-overproduced membranes efficiently catalyze a conversion of exogenous ferrous protoheme and farnesyl diphosphate to heme in the presence of divalent cations such as Mg2+ or Ca2+. The CyoE protein in vitro catalyzes a direct transfer of the farnesyl moiety from a farnesyl diphosphate-Mg2+ complex to position 2 of the vinyl group at pyrrole ring D of ferrous protoheme M, possibly by a one-step reaction Escherichia coli heme o + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.141 ? x * 26000, SDS-PAGE Escherichia coli
2.5.1.141 ? x * 28000, urea-SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.5.1.141 cyoE
-
 Escherichia coli
2.5.1.141 heme B farnesyltransferase
-
 Escherichia coli
2.5.1.141 heme o synthase
-
 Escherichia coli
2.5.1.141 JW0418 locus name Escherichia coli