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Literature summary extracted from

  • Sadowski, P.; Bakyta, I.
    T4 endonuclease IV. Improved purification procedure and resolution from T4 endonuclease 3 (1972), J. Biol. Chem., 247, 405-412.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.9 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.9
-
 Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.9 840000
-
 pH 7.5, 37°C Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.9 additional information enzyme displays about 200fold greater activity toward single stranded DNA than toward duplex DNA. The limit product obtained by exhaustive digestion of single stranded DNA with endonuclease IV is 25% acid-soluble and consists of oligonucleotides with an average chain length of about 50 nucleotides. Enzyme does not display exonuclease activity. No substrate: denatured bacteriophage T4 DNA Tequatrovirus T4 ?
-
 ?
3.1.21.9 phage fd DNA + H2O
-
 Tequatrovirus T4 ?
-
 ?
3.1.21.9 polydeoxycytydilate + H2O
-
 Tequatrovirus T4 ? product is acid-soluble material ending excluively in 5'-dCMP ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.9 8.1
-
-
 Tequatrovirus T4

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.21.9 7.4
-
 62% of maximum activity Tequatrovirus T4
3.1.21.9 8.5
-
 62% of maximum activity Tequatrovirus T4