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Literature summary extracted from

  • Yamano, S.; Sako, Y.; Nomura, N.; Maruyama, T.
    A cambialistic SOD in a strictly aerobic hyperthermophilic archaeon, Aeropyrum pernix (1999), J. Biochem., 126, 218-225.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 expression in Escherichia coli Aeropyrum pernix

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2 inactivates the Fe-reconstituted SOD in a time-dependent manner, but not the Mn-reconstituted enzyme. The incubation time for 50% inactivation of the Fe-reconstituted enzyme in the presence of 0.24 mM H2O2 is 50 min Aeropyrum pernix
1.15.1.1 Sodium azide 50% inhibition of the Fe-reconstituted enzyme at 41 mM. Sodium azide does not inhibit the Mn-reconstituted superoxide dismutase even at concentrations up to 400 mM Aeropyrum pernix
1.15.1.1 Sodium fluoride inhibits both the Mn- and Fe-reconstituted enzyme. The concentrations of sodium fluoride causing 50% inhibition of the Mn- and Fe reconstituted enzymes are 89 and 13 mM, respectively Aeropyrum pernix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ the enzyme contains both Mn and Fe. It is cambialistic, i.e. active with either Fe2+ or Mn2+. The specific activities were 906 U/mg with Mn2+ and 175 U/mg with Fe2+ Aeropyrum pernix
1.15.1.1 Mn2+ the enzyme contains both Mn and Fe. It is cambialistic, i.e. active with either Fe2+ or Mn2+. The specific activities were 906 U/mg with Mn2+ and 175 U/mg with Fe2+ Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 24600
-
 2 * 24600, calculated from sequence Aeropyrum pernix
1.15.1.1 60000
-
 gel filtration Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Aeropyrum pernix Q9Y8H8
-
-
1.15.1.1 Aeropyrum pernix DSM 11879 Q9Y8H8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1
-
 Aeropyrum pernix

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 24600, calculated from sequence Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 105
-
 5 h, no loss of activity Aeropyrum pernix
1.15.1.1 110
-
 5 h, 44% loss of activity Aeropyrum pernix