Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, T.T.; Zhou, N.Y.
    Novel L-cysteine-dependent maleylpyruvate isomerase in the gentisate pathway of Paenibacillus sp. strain NyZ101 (2012), J. Bacteriol., 194, 3987-3994.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.2.1.4 gene bagL, DNA and amino acid sequence determination and analysis, phylogenetic analysis, comparison of genetic organization of gentisate catabolic gene clusters, recombinant expression of the N-terminall His6-tagged enzyme in Escherichia coli strain BL21(DE3) Paenibacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.2.1.4 Ni2+ activates Paenibacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.2.1.4 3-Maleylpyruvate Paenibacillus sp.
-
3-Fumarylpyruvate
-
?
5.2.1.4 3-Maleylpyruvate Paenibacillus sp. NyZ101
-
3-Fumarylpyruvate
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.4 Paenibacillus sp. H6TG59 gene bagL
-
5.2.1.4 Paenibacillus sp. NyZ101 H6TG59 gene bagL
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.2.1.4 recombinant His6-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.4 3-Maleylpyruvate
-
Paenibacillus sp. 3-Fumarylpyruvate
-
?
5.2.1.4 3-Maleylpyruvate
-
Paenibacillus sp. NyZ101 3-Fumarylpyruvate
-
?
5.2.1.4 additional information BagL is a thiol-dependent maleylpyruvate isomerase catalyzing the isomerization of maleylpyruvate to fumarylpyruvate with L-cysteine, cysteinylglycine, or glutathione, as its cofactor Paenibacillus sp. ?
-
?
5.2.1.4 additional information BagL is a thiol-dependent maleylpyruvate isomerase catalyzing the isomerization of maleylpyruvate to fumarylpyruvate with L-cysteine, cysteinylglycine, or glutathione, as its cofactor Paenibacillus sp. NyZ101 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.2.1.4 homodimer
-
Paenibacillus sp.
5.2.1.4 More sequence analysis and homology modeling Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
5.2.1.4 BagL
-
Paenibacillus sp.
5.2.1.4 thiol-dependent maleylpyruvate isomerase
-
Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.2.1.4 30
-
assay at Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2.1.4 8
-
assay at Paenibacillus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
5.2.1.4 additional information L-cysteine, cysteinylglycine, or glutathione are possible cofactor, dependent on, cofactor kinetics, overview Paenibacillus sp.

Expression

EC Number Organism Comment Expression
5.2.1.4 Paenibacillus sp. the bag cluster is highly transcribed in 3-hydroxybenzoateinduced cells of strain NyZ101 up

General Information

EC Number General Information Comment Organism
5.2.1.4 evolution BagL is a L-cysteine-dependent catabolic enzyme in microbial metabolism and is distinct member of DinB family, with a four-helix-bundle topology Paenibacillus sp.