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Literature summary extracted from

  • Meng, Y.; Ingram-Smith, C.; Cooper, L.L.; Smith, K.S.
    Characterization of an archaeal medium-chain acyl coenzyme A synthetase from Methanosarcina acetivorans (2010), J. Bacteriol., 192, 5982-5990.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.2 heterologous production in Escherichia coli Methanosarcina acetivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.2 4.09
-
 CoA pH 7.5, 55°C Methanosarcina acetivorans
6.2.1.2 4.21
-
 ATP pH 7.5, 55°C Methanosarcina acetivorans
6.2.1.2 8.92
-
 2-methylbutyrate pH 7.5, 55°C Methanosarcina acetivorans

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.2 Methanosarcina acetivorans Q8TLW1
-
-
6.2.1.2 Methanosarcina acetivorans DSM 2834 Q8TLW1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.2
-
 Methanosarcina acetivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.2 ATP + 2-methylbutyrate + CoA when propionate or other less favorable acyl substrates, such as butyrate, 2-methylpropionate, or 2-methylvalerate, are utilized, the acyl-CoA is not produced or is produced at reduced levels. Instead, acyl-AMP and diphosphate are released in the absence of CoA, whereas in the presence of CoA, the intermediate is broken down into AMP and the acyl substrate, which are released along with diphosphate. These results suggest that although acyl-CoA synthetases may have the ability to utilize a broad range of substrates for the acyl-adenylate-forming first step of the reaction, the intermediate may not be suitable for the thioester-forming second step Methanosarcina acetivorans AMP + diphosphate + 2-methylbutyryl-CoA
-
 ?
6.2.1.2 ATP + 2-methylbutyrate + CoA when propionate or other less favorable acyl substrates, such as butyrate, 2-methylpropionate, or 2-methylvalerate, are utilized, the acyl-CoA is not produced or is produced at reduced levels. Instead, acyl-AMP and diphosphate are released in the absence of CoA, whereas in the presence of CoA, the intermediate is broken down into AMP and the acyl substrate, which are released along with diphosphate. These results suggest that although acyl-CoA synthetases may have the ability to utilize a broad range of substrates for the acyl-adenylate-forming first step of the reaction, the intermediate may not be suitable for the thioester-forming second step Methanosarcina acetivorans DSM 2834 AMP + diphosphate + 2-methylbutyryl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.2 Macs
-
 Methanosarcina acetivorans
6.2.1.2 medium-chain acyl coenzyme A synthetase
-
 Methanosarcina acetivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.2 55
-
 assay at Methanosarcina acetivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.2 1.58
-
 2-methylbutyrate pH 7.5, 55°C Methanosarcina acetivorans
6.2.1.2 1.94
-
 ATP pH 7.5, 55°C Methanosarcina acetivorans
6.2.1.2 2.15
-
 CoA pH 7.5, 55°C Methanosarcina acetivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.2 7.5
-
 assay at Methanosarcina acetivorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.2 0.18
-
 2-methylbutyrate pH 7.5, 55°C Methanosarcina acetivorans
6.2.1.2 0.46
-
 ATP pH 7.5, 55°C Methanosarcina acetivorans
6.2.1.2 0.53
-
 CoA pH 7.5, 55°C Methanosarcina acetivorans