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Literature summary extracted from

  • Bowen, T.L.; Lin, W.C.; Whitman, W.B.
    Characterization of guanine and hypoxanthine phosphoribosyltransferases in Methanococcus voltae (1996), J. Bacteriol., 178, 2521-2526.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.8 GMP 0.05 mM, at least 2fold stimulation, hypoxanthine phosphoribosyltransferase activity Methanococcus voltae
2.4.2.8 IMP 0.2 mM, at least 2fold stimulation, hypoxanthine phosphoribosyltransferase activity Methanococcus voltae
2.4.2.8 additional information hypoxanthine phosphoribosyltransferase activity is unaffected by AMP Methanococcus voltae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.8 GMP 0.3 mM, 50% inhibition of guanine phosphoribosyltransferase activity Methanococcus voltae
2.4.2.8 guanine 0.06 mM, 50% inhibition of hypoxanthine phosphoribosyltransferase activity Methanococcus voltae
2.4.2.8 hypoxanthine 0.16 mM, 50% inhibition of guanine phosphoribosyltransferase activity Methanococcus voltae
2.4.2.8 additional information guanine phosphoribosyltransferase activity: IMP and AMP are not inhibitory at concentrations up to 0.6 mM, adenine and xanthine are not inhibitory at concentrations up to 0.5 mM. Adenine and xanthine do not inhibit hypoxanthine phosphoribosyltransferase activity Methanococcus voltae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.015
-
 hypoxanthine pH 9.0, 37°C Methanococcus voltae
2.4.2.8 0.2
-
 guanine pH 9.0, 37°C Methanococcus voltae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Methanococcus voltae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 0.00085
-
 pH 9.0, 37°C, hypoxanthine phosphoribosyltransferase activity Methanococcus voltae
2.4.2.8 0.0027
-
 pH 9.0, 37°C, guanine phosphoribosyltransferase activity Methanococcus voltae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Methanococcus voltae GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Methanococcus voltae IMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 guanine PRTase
-
 Methanococcus voltae
2.4.2.8 hypoxanthine PRTase
-
 Methanococcus voltae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 9
-
 guanine phosphoribosyltransferase activity and hypoxanthine phosphoribosyltransferase activity Methanococcus voltae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.8 7.5 10 half-maximal activity at pH 7.5, and 10.0, guanine phosphoribosyltransferase activity Methanococcus voltae
2.4.2.8 7.5 9.5 half-maximal activity at pH 7.5, and 9.5, hypoxanthine phosphoribosyltransferase activity Methanococcus voltae