EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.3.2 | 0.0508 | - |
hypoxanthine | pH 8.5, 43°C | Blastobotrys adeninivorans | |
1.17.3.2 | 0.0728 | - |
xanthine | pH 8.5, 43°C | Blastobotrys adeninivorans | |
1.17.3.2 | 0.162 | - |
NAD+ | pH 8.5, 43°C | Blastobotrys adeninivorans | |
1.17.3.2 | 0.208 | - |
purine | pH 8.5, 43°C | Blastobotrys adeninivorans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.3.2 | Cd2+ | inhibitory effect | Blastobotrys adeninivorans | |
1.17.3.2 | Cu2+ | strong inhibition | Blastobotrys adeninivorans | |
1.17.3.2 | Fe2+ | inhibitory effect | Blastobotrys adeninivorans | |
1.17.3.2 | Zn2+ | inhibitory effect | Blastobotrys adeninivorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 160000 | - |
SDS-PAGE | Blastobotrys adeninivorans |
1.17.3.2 | 360000 | - |
gel filtration | Blastobotrys adeninivorans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.3.2 | Blastobotrys adeninivorans | R4ZGN4 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.3.2 | hypoxanthine + NAD+ + H2O | - |
Blastobotrys adeninivorans | xanthine + NADH + H+ | - |
? | |
1.17.3.2 | pterine + NAD+ | - |
Blastobotrys adeninivorans | ? + H2O + NADH | - |
? | |
1.17.3.2 | purine + NAD+ | - |
Blastobotrys adeninivorans | ? + H2O + NADH | - |
? | |
1.17.3.2 | xanthine + H2O + O2 | - |
Blastobotrys adeninivorans | urate + H2O2 | - |
? | |
1.17.3.2 | xanthine + NAD+ + H2O | - |
Blastobotrys adeninivorans | urate + NADH | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.3.2 | AXOR | - |
Blastobotrys adeninivorans |
1.17.3.2 | xanthine oxidoreductase | - |
Blastobotrys adeninivorans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 43 | - |
- |
Blastobotrys adeninivorans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 35 | 50 | - |
Blastobotrys adeninivorans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 30 | - |
5% activity in 1 h at 30°C | Blastobotrys adeninivorans |
1.17.3.2 | 40 | - |
12% activity in 1 h at 40°C | Blastobotrys adeninivorans |
1.17.3.2 | 50 | - |
22% activity in 1 h at 50°C | Blastobotrys adeninivorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 8.5 | - |
glycine-NaOH buffer | Blastobotrys adeninivorans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 7.5 | 9.5 | potassium phosphate, Tris-HCl, Tris-acetate and glycine-NaOH buffer | Blastobotrys adeninivorans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.17.3.2 | malfunction | deletion mutant exhibits less than 1% of the wild-type strain activity towards xanthine and hypoxanthine under inducible conditions and both strains show no activity after cultivation without inducer (adenine) | Blastobotrys adeninivorans |