EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.81 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.81 | 0.000154 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis | |
4.1.2.25 | 0.00016 | - |
7,8-dihydroneopterin | pH 7.0, 22°C | Mycobacterium tuberculosis | |
4.1.2.25 | 0.00017 | - |
7,8-dihydromonapterin | pH 7.0, 22°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.25 | additional information | no metal required | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.81 | 16585 | - |
4 * 16585, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
1.13.11.81 | 16716 | - |
4 * 16716, calculated from amino acid sequence | Mycobacterium tuberculosis |
1.13.11.81 | 62300 | - |
sedimentation equilibrium analysis | Mycobacterium tuberculosis |
4.1.2.25 | 16585 | - |
apo-enzyme, 4 * 16585, and octamer for 7,8-dihydroxanthopterin-bound form, 8 * 16585, mass spectrometry | Mycobacterium tuberculosis |
4.1.2.25 | 62300 | - |
apo-enzyme, mass spectrometry | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.81 | 7,8-dihydroneopterin + O2 | Mycobacterium tuberculosis | - |
7,8-dihydroxanthopterin + formate + glycolaldehyde | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.81 | Mycobacterium tuberculosis | P9WNC5 | - |
- |
4.1.2.25 | Mycobacterium tuberculosis | P9WNC5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.81 | Ni-NTA column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.81 | 7,8-dihydroneopterin + O2 | - |
Mycobacterium tuberculosis | 7,8-dihydroxanthopterin + formate + glycolaldehyde | - |
r | |
4.1.2.25 | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
Mycobacterium tuberculosis | 7,8-dihydroneopterin + 7,8-dihydromonapterin | reaction is reversible, and C-C bond formation has both 7,8-dihydroneopterin and 7,8-dihydromonapterin as products plus 7,8-dihydroxanthopterin | r | |
4.1.2.25 | 7,8-dihydromonapterin | - |
Mycobacterium tuberculosis | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
r | |
4.1.2.25 | 7,8-dihydroneopterin | - |
Mycobacterium tuberculosis | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
r | |
4.1.2.25 | additional information | enzyme can utilize both dihydroneopterin and 7,8-dihydromonapterin as substrates to generate 6-hydroxymethyl-7,8-dihydropterin. The reaction generates three different pterin products, one of which is not produced by other wild-type dihydroneopterin aldolases. The enzyme-substrate complex partitions 51% in the first turnover to form the aldolase products, 24% to the epimerase product and 25% to the oxygenase products. The aldolase reaction is strongly pH dependent. Chemistry is rate limiting for the aldolase reaction, and two protons and a likely solvent contribution are involved in formation and breakage of a common intermediate | Mycobacterium tuberculosis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.81 | homotetramer | 4 * 16585, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
1.13.11.81 | homotetramer | 4 * 16716, calculated from amino acid sequence | Mycobacterium tuberculosis |
4.1.2.25 | tetramer | apo-enzyme, 4 * 16585, and octamer for 7,8-dihydroxanthopterin-bound form, 8 * 16585, mass spectrometry | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.81 | DHNA | - |
Mycobacterium tuberculosis |
1.13.11.81 | dihydroneopterin oxygenase | - |
Mycobacterium tuberculosis |
1.13.11.81 | Rv3607c | - |
Mycobacterium tuberculosis |
4.1.2.25 | DHNA | - |
Mycobacterium tuberculosis |
4.1.2.25 | FolB | - |
Mycobacterium tuberculosis |
4.1.2.25 | Rv3607c | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.81 | 0.0011 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis | |
4.1.2.25 | 0.0054 | - |
7,8-dihydroneopterin | pH 7.0, 22°C | Mycobacterium tuberculosis | |
4.1.2.25 | 0.006 | - |
7,8-dihydromonapterin | pH 7.0, 22°C | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.81 | 7.3 | - |
7,8-dihydroneopterin | at pH 7.0 and 25°C | Mycobacterium tuberculosis | |
4.1.2.25 | 36 | - |
7,8-dihydroneopterin | pH 7.0, 22°C | Mycobacterium tuberculosis | |
4.1.2.25 | 38 | - |
7,8-dihydromonapterin | pH 7.0, 22°C | Mycobacterium tuberculosis |