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Literature summary extracted from

  • Mahanty, S.; Kaul, T.; Pandey, P.; Reddy, R.A.; Mallikarjuna, G.; Reddy, C.S.; Sopory, S.K.; Reddy, M.K.
    Biochemical and molecular analyses of copper-zinc superoxide dismutase from a C4 plant Pennisetum glaucum reveals an adaptive role in response to oxidative stress (2012), Gene, 505, 309-317.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Cenchrus americanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2 50% inhibition at 4.3 mM Cenchrus americanus
1.15.1.1 additional information no inhibition by 10 mM NaN3 Cenchrus americanus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.15.1.1 cytosol
-
 Cenchrus americanus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a CuZn-SOD Cenchrus americanus
1.15.1.1 Zn2+ a CuZn-SOD Cenchrus americanus

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Cenchrus americanus A4ZYP8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to near homogeneity Cenchrus americanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 seedling
-
 Cenchrus americanus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 additional information SOD activity is assayed based on its ability to compete with nitroblue-tetrazolium for superoxide anions generated by the xanthine-xanthine oxidase system, which in turn results in the inhibition of reduction of nitroblue-tetrazolium Cenchrus americanus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 More homology structure modeling of PgCuZnSOD, overview Cenchrus americanus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 copper-zinc superoxide dismutase
-
 Cenchrus americanus
1.15.1.1 CuZnSOD
-
 Cenchrus americanus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 44.5
-
 purified recombinant His-tagged enzyme, 60 min, loss of 50% activity Cenchrus americanus
1.15.1.1 70
-
 purified recombinant His-tagged enzyme, 60 min, loss of 96% activity Cenchrus americanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.6
-
-
 Cenchrus americanus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 7 10 maximal activity at pH 7.6, 50% of miximal activity at pH 8.5, inactivation above pH 10.0 Cenchrus americanus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Cenchrus americanus isoelectric focusing
-
 5.76

Expression

EC Number Organism Comment Expression
1.15.1.1 Cenchrus americanus in Pennisetum seedlings, abiotic stress-induced PgCuZnSOD transcript upregulation directly correlates to high protein and activity induction. PgCuZnSOD mRNA levels gradually increase to several folds on exposure of Pennisetum seedlings corresponding to gradual increase in the time period of different stress treatments i.e. dehydration, methyl viologen, NaCl and high temperature (48°C) with an exception in cold stress (4°C) where the transcript initially increased (0-12 h time-point) in comparison to control and later decreased (24 h time-point) with regards to initial rise up

General Information

EC Number General Information Comment Organism
1.15.1.1 additional information overexpression of PgCuZnSOD confers comparatively enhanced tolerance to methyl viologen induced oxidative stress in bacteria. Homology structure modeling of PgCuZnSOD, overview Cenchrus americanus
1.15.1.1 physiological function PgCuZnSOD plays a functional role in conferring oxidative stress tolerance to prokaryotic system Cenchrus americanus