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Literature summary extracted from

  • Liu, N.N.; Liu, W.; Wang, D.J.; Zhou, Y.B.; Lin, X.J.; Wang, X.; Li, S.B.
    Purification and partial characterization of polyphenol oxidase from the flower buds of Lonicera japonica Thunb (2013), Food Chem., 138, 478-483.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.1 SDS 170% activity at 10 mM Lonicera japonica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 ascorbic acid nearly complete inhibition at 10 mM Lonicera japonica
1.10.3.1 Citric acid 32% residual activity at 10 mM Lonicera japonica
1.10.3.1 Co2+ 35% residual activity at 10 mM Lonicera japonica
1.10.3.1 Cu2+ 33% residual activity at 10 mM Lonicera japonica
1.10.3.1 kojic acid 11% residual activity at 10 mM Lonicera japonica
1.10.3.1 Mg2+ inhibits activity at 0.01 mM Lonicera japonica
1.10.3.1 Mn2+ inhibits activity at 0.01 mM Lonicera japonica
1.10.3.1 additional information not inhibited by SDS Lonicera japonica
1.10.3.1 Sodium metabisulfite 27% residual activity at 10 mM Lonicera japonica
1.10.3.1 tropolone 34% residual activity at 10 mM Lonicera japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 0.213
-
 catechol at pH 7.5 and 25°C Lonicera japonica
1.10.3.1 1
-
 chlorogenic acid at pH 7.5 and 25°C Lonicera japonica
1.10.3.1 2.69
-
 L-Dopa at pH 7.5 and 25°C Lonicera japonica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.1 Ca2+ 124% activity at 10 mM Lonicera japonica
1.10.3.1 copper a copper-containing enzyme with a dinuclear copper centre Lonicera japonica
1.10.3.1 Mg2+ stimulates activity to 112% at 10 mM Lonicera japonica
1.10.3.1 Mn2+ stimulates activity to 120% at 10 mM Lonicera japonica
1.10.3.1 additional information Na+, K+, Cl- and SO42- have no effect on enzyme activity Lonicera japonica
1.10.3.1 Zn2+ 122% activity at 0.1 mM Lonicera japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 49000
-
 1 * 49000, SDS-PAGE Lonicera japonica
1.10.3.1 49277
-
 1 * 49277, LC-ESI mass spectrometry Lonicera japonica

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Lonicera japonica
-
 variety Yate
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 ammonium sulfate precipitation and DEAE-cellulose column chromatography Lonicera japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 flower bud
-
 Lonicera japonica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 0.145
-
 using L-DOPA as substrate, crude enzyme, at pH 7.5 and 25°C Lonicera japonica
1.10.3.1 4.458
-
 using L-DOPA as substrate, after 30.76fold purification, at pH 7.5 and 25°C Lonicera japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 catechol + O2 best substrate Lonicera japonica ?
-
 ?
1.10.3.1 chlorogenic acid + O2
-
 Lonicera japonica ?
-
 ?
1.10.3.1 L-DOPA + O2
-
 Lonicera japonica ?
-
 ?
1.10.3.1 additional information polyphenol oxidase shows no activity with L-tyrosine, digallol, gallic acid, and methyl gallate Lonicera japonica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 monomer 1 * 49000, SDS-PAGE Lonicera japonica
1.10.3.1 monomer 1 * 49277, LC-ESI mass spectrometry Lonicera japonica

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 polyphenol oxidase
-
 Lonicera japonica
1.10.3.1 PPO
-
 Lonicera japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.1 25
-
-
 Lonicera japonica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 40
-
 the residual soluble enzyme activity decreases significantly when temperature reaches 40°C Lonicera japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 7.5
-
-
 Lonicera japonica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.1 3 10 the enzyme is stable within pH 8.0-10.0, retaining more than 80% of its activity. The enzyme is almost unstable and inactive when pH is below 3.0, only remaining less than 20% of its activity Lonicera japonica