Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Iwabata, H.; Watanabe, K.; Ohkuri, T.; Yokobori, S.; Yamagishi, A.
    Thermostability of ancestral mutants of Caldococcus noboribetus isocitrate dehydrogenase (2005), FEMS Microbiol. Lett., 243, 393-398.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 wild-type and mutant enzymes are expressed in Escherichia coli Caldococcus noboribetus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 A325P/G326S 50% inactivation after 10 min at 91.3°C as compared to 87.5°C for wild-type enzyme Caldococcus noboribetus
1.1.1.42 A336F 50% inactivation after 10 min at 74°C as compared to 87.5°C or wild-type enzyme Caldococcus noboribetus
1.1.1.42 I321L 50% inactivation after 10 min at 88.9°C as compared to 87.5°C for wild-type enzyme Caldococcus noboribetus
1.1.1.42 Y309I/I310L 50% inactivation after 10 min at 88.4°C as compared to 87.5°C for wild-type enzyme Caldococcus noboribetus
1.1.1.42 Y309I/I310L/I321L/A325P/G326S 50% inactivation after 10 min at 90°C as compared to 87.5°C for wild-type enzyme Caldococcus noboribetus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Caldococcus noboribetus
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Caldococcus noboribetus P96318
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 wild-type and mutant enzymes Caldococcus noboribetus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Caldococcus noboribetus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 ICDH
-
 Caldococcus noboribetus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 70
-
 assay at Caldococcus noboribetus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 74
-
 10 min, 50% loss of activity, mutant enzyme A336F Caldococcus noboribetus
1.1.1.42 88
-
 10 min, 50% loss of activity, wild-type enzyme and mutant enzyme Y309I/I310L Caldococcus noboribetus
1.1.1.42 89
-
 10 min, 50% loss of activity, mutant enzyme I321L Caldococcus noboribetus
1.1.1.42 91
-
 10 min, 50% loss of activity, mutant enzyme A325P/G326S Caldococcus noboribetus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7
-
 assay at Caldococcus noboribetus