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Literature summary extracted from

  • Ronimus, R.S.; Kawarabayasi, Y.; Kikuchi, H.; Morgan, H.W.
    Cloning, expression and characterisation of a Family B ATP-dependent phosphofructokinase activity from the hyperthermophilic crenarachaeon Aeropyrum pernix (2001), FEMS Microbiol. Lett., 202, 85-90.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.11 expression in Escherichia coli Aeropyrum pernix

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.11 ADP 1 mM, 82% inhibition Aeropyrum pernix
2.7.1.11 Cu2+ 1 mM, 52.4% inhibition Aeropyrum pernix
2.7.1.11 diphosphate 1 mM, 21% inhibition Aeropyrum pernix
2.7.1.11 GDP 1 mM, 46% inhibition Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.11 0.027
-
 ATP pH 6.5, 95°C, discontinuous assay Aeropyrum pernix
2.7.1.11 1.212
-
 D-fructose 6-phosphate pH 6.5, 95°C, discontinuous assay Aeropyrum pernix

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.11 KCl optimal activity between 50 and 100 mM. 85% of maximal activity when the KCl concentration is 500 mM Aeropyrum pernix
2.7.1.11 Mg2+ the optimal MgCl2 concentration for activity is 20-30 mM and the enzyme possesses 6, 36 and 74% activity compared to the maximal activity when the MgCl2 concentrations are 1.25, 2.5 and 5.0 mM, respectively Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.11 29000
-
 gel filtration Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.11 Aeropyrum pernix Q9YG89
-
-
2.7.1.11 Aeropyrum pernix DSM 11879 Q9YG89
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Aeropyrum pernix ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 ATP + D-fructose 6-phosphate
-
 Aeropyrum pernix DSM 11879 ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 CTP + D-fructose 6-phosphate 20.6% of the activity with ATP Aeropyrum pernix CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 CTP + D-fructose 6-phosphate 20.6% of the activity with ATP Aeropyrum pernix DSM 11879 CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate 84.1% of the activity with ATP Aeropyrum pernix GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.11 GTP + D-fructose 6-phosphate 84.1% of the activity with ATP Aeropyrum pernix DSM 11879 GDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.11 monomer 1 * 29000, SDS-PAGE Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
2.7.1.11 APE0012
-
 Aeropyrum pernix
2.7.1.11 ATP-PFK
-
 Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.11 95
-
 assay at Aeropyrum pernix

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.11 85 95 85°C: 50% of maximal activity, 95°C: optimum Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.11 95
-
 half-life: 30 min Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.11 5.5 6.5
-
 Aeropyrum pernix

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.11 5.5 7.5 the pH optimum for activity of the enzyme is analysed only between the pH values of 5.5 to 7.5 due to concerns about instability of the ATP at more acid pH values and is found to be maximal between 5.5 and 6.5, but possesses 69% of maximal activity at pH 7.5 Aeropyrum pernix