Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Foster, J.; Nakata, P.A.
    An oxalyl-CoA synthetase is important for oxalate metabolism in Saccharomyces cerevisiae (2014), FEBS Lett., 588, 160-166.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.8 gene YBR222C, expression of His-tagged enzyme in Escherichia coli strain BLR(DE3) Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.8 additional information
-
 additional information Michaelis-Menten kinetics Saccharomyces cerevisiae
6.2.1.8 0.02
-
 oxalate pH 7.5, temperature not specified in the publication Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.8 ATP + oxalate + CoA Saccharomyces cerevisiae
-
 AMP + diphosphate + oxalyl-CoA
-
 ?
6.2.1.8 ATP + oxalate + CoA Saccharomyces cerevisiae BY4741
-
 AMP + diphosphate + oxalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.8 Saccharomyces cerevisiae P38137 gene YBR222C
-
6.2.1.8 Saccharomyces cerevisiae BY4741 P38137 gene YBR222C
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.8 recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography to over 90% purity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.8 ATP + oxalate + CoA
-
 Saccharomyces cerevisiae AMP + diphosphate + oxalyl-CoA
-
 ?
6.2.1.8 ATP + oxalate + CoA
-
 Saccharomyces cerevisiae BY4741 AMP + diphosphate + oxalyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.8 acyl-activating enzyme 3
-
 Saccharomyces cerevisiae
6.2.1.8 Oxalyl-CoA synthetase
-
 Saccharomyces cerevisiae
6.2.1.8 ScAAE3
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.8 7.5
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.8 6 10 activity range, profile overview Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.8 ATP
-
 Saccharomyces cerevisiae

Expression

EC Number Organism Comment Expression
6.2.1.8 Saccharomyces cerevisiae oxalate induced the enzyme, quantitative RT-PCR reveals that ScAAE3 transcript levels are induced within min of exposure peaking at about 1 h before declining back to steady state levels up

General Information

EC Number General Information Comment Organism
6.2.1.8 malfunction a Scaae3 deletion mutant strain shows highly reduced oxalate degradation activity decreasing recovery from exposure to oxalate, e.g. by exposure to oxalate-secreting microbes, and to oxidative stress, e.g. by H2O2 Saccharomyces cerevisiae
6.2.1.8 physiological function the enzyme catalyzes the first step in a pathway of oxalate degradation to protect the cell against the harmful effects of oxalate derived from an endogenous process or an environmental source Saccharomyces cerevisiae