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Literature summary extracted from

  • Lee, Y.J.; Lee, S.J.; Kim, S.B.; Lee, S.J.; Lee, S.H.; Lee, D.W.
    Structural insights into conserved L-arabinose metabolic enzymes reveal the substrate binding site of a thermophilic L-arabinose isomerase (2014), FEBS Lett., 588, 1064-1070.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.4 expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.4 D118V the mutant shows 42% of wild type activity Geobacillus stearothermophilus
5.3.1.4 D195V the mutant shows 29% of wild type activity Geobacillus stearothermophilus
5.3.1.4 D309V the mutant shows 78% of wild type activity Geobacillus stearothermophilus
5.3.1.4 D333V the mutant shows 40% of wild type activity Geobacillus stearothermophilus
5.3.1.4 E133L the mutant shows 65% of wild type activity Geobacillus stearothermophilus
5.3.1.4 E233L the mutant shows wild type activity Geobacillus stearothermophilus
5.3.1.4 E261L the mutant shows 29% of wild type activity Geobacillus stearothermophilus
5.3.1.4 E332L the mutant shows 67% of wild type activity Geobacillus stearothermophilus
5.3.1.4 K196F the mutant shows 56% of wild type activity Geobacillus stearothermophilus
5.3.1.4 N406L the mutant shows 57% of wild type activity Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 42.7
-
 L-arabinose mutant enzyme D118V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 57.9
-
 L-arabinose wild type enzyme, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 80
-
 L-arabinose mutant enzyme N460L, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 100
-
 L-arabinose mutant enzyme D333V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 119.3
-
 L-arabinose mutant enzyme D195V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 147.1
-
 L-arabinose mutant enzyme E261L, at pH 7.5 and 70°C Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Mn2+ about 2fold increase in activity at 1 mM Mn2+ Geobacillus stearothermophilus
5.3.1.4 additional information not significantly stimulated by Mg2+ Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 L-arabinose Geobacillus stearothermophilus
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 His-bind resin column chromatography, and Superdex 200 gel filtration Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 L-arabinose
-
 Geobacillus stearothermophilus L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 homohexamer
-
 Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.4 11.2
-
 L-arabinose mutant enzyme E261L, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 11.9
-
 L-arabinose mutant enzyme D118V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 14.2
-
 L-arabinose mutant enzyme D195V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 14.9
-
 L-arabinose mutant enzyme D333V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 27.8
-
 L-arabinose mutant enzyme N460L, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 33.8
-
 L-arabinose wild type enzyme, at pH 7.5 and 70°C Geobacillus stearothermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.4 0.08
-
 L-arabinose mutant enzyme E261L, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 0.12
-
 L-arabinose mutant enzyme D195V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 0.15
-
 L-arabinose mutant enzyme D333V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 0.28
-
 L-arabinose mutant enzyme D118V, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 0.35
-
 L-arabinose mutant enzyme N460L, at pH 7.5 and 70°C Geobacillus stearothermophilus
5.3.1.4 0.58
-
 L-arabinose wild type enzyme, at pH 7.5 and 70°C Geobacillus stearothermophilus