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Literature summary extracted from

  • Ko, J.H.; Wang, Y.S.; Nakamura, A.; Guo, L.T.; Soell, D.; Umehara, T.
    Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function (2013), FEBS Lett., 587, 3243-3248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.26 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.26 A302F/Y306L/N346T/C348F/Y384L the mutant acylates tRNAPyl with L-phenylalanine Escherichia coli
6.1.1.26 A302L/Y306M/N346S/C348L/Y384L the mutant acylates tRNAPyl with L-phenylalanine Escherichia coli
6.1.1.26 N346A/C348L the mutant acylates tRNAPyl with L-phenylalanine Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.26 1 4 L-phenylalanine mutant enzyme A302L/Y306M/N346S/C348L/Y384L, pH and temperature not specified in the publication Escherichia coli
6.1.1.26 21
-
 L-phenylalanine mutant enzyme N346A/C348L, pH and temperature not specified in the publication Escherichia coli
6.1.1.26 22.8
-
 L-phenylalanine mutant enzyme A302F/Y306L/N346T/C348F/Y384L, pH and temperature not specified in the publication Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl Escherichia coli
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 additional information Escherichia coli the wild type enzyme shows no activity with L-phenylalanine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.26 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.26 ATP + L-phenylalanine + tRNAPyl mutant enzymes N346A/C348L, A302L/Y306M/N346S/C348L/Y384L, and A302F/Y306L/N346T/C348F/Y384L use L-phenylalanine as substrate Escherichia coli AMP + diphosphate + L-phenylalanyl-tRNAPyl
-
 ?
6.1.1.26 ATP + L-pyrrolysine + tRNAPyl
-
 Escherichia coli AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
6.1.1.26 additional information the wild type enzyme shows no activity with L-phenylalanine Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.26 PylRS
-
 Escherichia coli
6.1.1.26 pyrrolysyl-tRNA synthetase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.26 0.037
-
 L-phenylalanine mutant enzyme N346A/C348L, pH and temperature not specified in the publication Escherichia coli
6.1.1.26 0.062
-
 L-phenylalanine mutant enzyme A302L/Y306M/N346S/C348L/Y384L, pH and temperature not specified in the publication Escherichia coli
6.1.1.26 0.075
-
 L-phenylalanine mutant enzyme A302F/Y306L/N346T/C348F/Y384L, pH and temperature not specified in the publication Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.26 ATP
-
 Escherichia coli