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Literature summary extracted from

  • Nagatoshi, M.; Terasaka, K.; Owaki, M.; Sota, M.; Inukai, T.; Nagatsu, A.; Mizukami, H.
    UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides (2012), FEBS Lett., 586, 1055-1061.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.330 beta-D-glucosyl crocetin above 0.2 mM, substrate inhibition Gardenia jasminoides
2.4.1.330 additional information no substrate inhibition with bis(beta-D-glucosyl) crocetin Gardenia jasminoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.330 0.023
-
 bis(beta-D-glucosyl) crocetin pH 7.5, 30°C Gardenia jasminoides
2.4.1.330 0.023
-
 bis(beta-D-glucosyl) crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides
2.4.1.330 0.072
-
 beta-D-glucosyl crocetin pH 7.5, 30°C Gardenia jasminoides
2.4.1.330 0.072
-
 beta-D-glucosyl crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.330 49800
-
 calculated from sequence Gardenia jasminoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.330 UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin Gardenia jasminoides the enzyme is involved in biosynthesis of crocin UDP + crocin
-
 ?
2.4.1.330 UDP-alpha-D-glucose + beta-D-glucosyl crocetin Gardenia jasminoides the enzyme is involved in biosynthesis of crocin UDP + beta-D-gentiobiosyl crocetin
-
 ?
2.4.1.330 UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin Gardenia jasminoides the enzyme is involved in biosynthesis of crocin UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.330 Gardenia jasminoides F8WKW8
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.330 UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin (3) Gardenia jasminoides
a
2.4.1.330 UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin (1) Gardenia jasminoides
a
2.4.1.330 UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin (2) Gardenia jasminoides
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.330 additional information mRNA expression is etected in all organs tested Gardenia jasminoides
-
2.4.1.330 seedling cultured cells obtained from seedlings Gardenia jasminoides
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.330 additional information no activity with crocetin Gardenia jasminoides ?
-
 ?
2.4.1.330 additional information enzyme does not show activity against various flavonoid glucosides and coumarin glucosides as well as unnatural glucosides such as curcumin glucosides and 4-nitrophenyl glucoside Gardenia jasminoides ?
-
 ?
2.4.1.330 UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
 Gardenia jasminoides UDP + crocin
-
 ?
2.4.1.330 UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin the enzyme is involved in biosynthesis of crocin Gardenia jasminoides UDP + crocin
-
 ?
2.4.1.330 UDP-alpha-D-glucose + beta-D-glucosyl crocetin
-
 Gardenia jasminoides UDP + beta-D-gentiobiosyl crocetin
-
 ?
2.4.1.330 UDP-alpha-D-glucose + beta-D-glucosyl crocetin the enzyme is involved in biosynthesis of crocin Gardenia jasminoides UDP + beta-D-gentiobiosyl crocetin
-
 ?
2.4.1.330 UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
-
 Gardenia jasminoides UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
 ?
2.4.1.330 UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin the enzyme is involved in biosynthesis of crocin Gardenia jasminoides UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.330 GjUGT9
-
 Gardenia jasminoides
2.4.1.330 UDP-glucose:crocetin glucosyl ester glucosyltransferase
-
 Gardenia jasminoides
2.4.1.330 UGT94E5
-
 Gardenia jasminoides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.330 30
-
 assay at Gardenia jasminoides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.330 0.036
-
 beta-D-glucosyl crocetin pH 7.5, 30°C Gardenia jasminoides
2.4.1.330 0.036
-
 beta-D-glucosyl crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides
2.4.1.330 0.071
-
 bis(beta-D-glucosyl) crocetin pH 7.5, 30°C Gardenia jasminoides
2.4.1.330 0.071
-
 bis(beta-D-glucosyl) crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.330 7.5
-
 assay at Gardenia jasminoides

General Information

EC Number General Information Comment Organism
2.4.1.330 metabolism the enzyme is involved in biosynthesis of crocin Gardenia jasminoides
2.4.1.330 physiological function glycosyl transferase UGT94E5 is involved in synhtesis of apocarotenoid glycosyl ester crocin accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical Gardenia jasminoides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.330 0.51
-
 beta-D-glucosyl crocetin pH 7.5, 30°C Gardenia jasminoides
2.4.1.330 0.51
-
 beta-D-glucosyl crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides
2.4.1.330 3.1
-
 bis(beta-D-glucosyl) crocetin pH 7.5, 30°C Gardenia jasminoides
2.4.1.330 3.1
-
 bis(beta-D-glucosyl) crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides