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Literature summary extracted from

  • Mine, S.; Niiyama, M.; Hashimoto, W.; Ikegami, T.; Koma, D.; Ohmoto, T.; Fukuda, Y.; Inoue, T.; Abe, Y.; Ueda, T.; Morita, J.; Uegaki, K.; Nakamura, T.
    Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N'-diacetylchitobiose deacetylase (2014), FEBS J., 281, 2584-2596.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.105 expression of the soluble recombinant protein in Escherichia coli Pyrococcus horikoshii
3.5.1.105 expression of the soluble recombinant protein in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.105 crystal structure is determined at resolutions of 1.54 A Pyrococcus furiosus
3.5.1.105 crystal structure is determined at resolutions of 2.0 A Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.105 Zinc
-
Pyrococcus furiosus
3.5.1.105 Zinc the catalytic zinc that is situated at the end of the intermolecular cleft is coordinated by three side chain ligands from His44, Asp47, and His155, and by a phosphate ion derived from the crystallization reservoir solution Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.105 Pyrococcus furiosus Q8U3V1
-
-
3.5.1.105 Pyrococcus horikoshii O58235
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.105
-
Pyrococcus horikoshii
3.5.1.105
-
Pyrococcus furiosus

Subunits

EC Number Subunits Comment Organism
3.5.1.105 hexamer comprised of two trimers. An intermolecular cleft is formed by two polypeptides in the trimeric assembly Pyrococcus horikoshii
3.5.1.105 hexamer comprised of two trimers. An intermolecular cleft is formed by two polypeptides in the trimeric assembly Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.105 N,N'-diacetylchitobiose deacetylase
-
Pyrococcus horikoshii
3.5.1.105 N,N'-diacetylchitobiose deacetylase
-
Pyrococcus furiosus
3.5.1.105 PF0354 locus name Pyrococcus furiosus
3.5.1.105 PH0499 locus name Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.105 85
-
thermostable up to Pyrococcus horikoshii
3.5.1.105 85
-
thermostable up to Pyrococcus furiosus