Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Castro-Fernandez, V.; Bravo-Moraga, F.; Herrera-Morande, A.; Guixe, V.
    Bifunctional ADP-dependent phosphofructokinase/glucokinase activity in the order Methanococcales - biochemical characterization of the mesophilic enzyme from Methanococcus maripaludis (2014), FEBS J., 281, 2017-2029.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.146 D-glucosamine 10 mM, 40% activation of glucokinase activity Methanococcus maripaludis
2.7.1.147 D-glucosamine 10 mM, 40% activation of glucokinase activity Methanococcus maripaludis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.146 expression in Escherichia coli Methanococcus maripaludis
2.7.1.147 expression in Escherichia coli Methanococcus maripaludis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.146 molecular modeling of structure. for binding of ADP, residues M347, I431 and L441 create a hydrophobic pocket around the adenine group. R194 makes a hydrogen bond with alpha and beta phosphates, carbonyl and NH groups from V432 peptide bond make a hydrogen bond with the NH2 group of C6 and the N1 atom of adenine Methanococcus maripaludis
2.7.1.147 molecular modeling of structure. for binding of ADP, residues M347, I431 and L441 create a hydrophobic pocket around the adenine group. R194 makes a hydrogen bond with alpha and beta phosphates, carbonyl and NH groups from V432 peptide bond make a hydrogen bond with the NH2 group of C6 and the N1 atom of adenine Methanococcus maripaludis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.146 ADP strong inhibition effect in the reverse glucokinase reaction Methanococcus maripaludis
2.7.1.146 AMP strong substrate inhibition effect in the reverse glucokinase reaction Methanococcus maripaludis
2.7.1.146 D-fructose
-
Methanococcus maripaludis
2.7.1.146 D-fructose 6-phosphate strong inhibition of glucokinase activity, competitive inhibion versus D-glucose as variable substrate Methanococcus maripaludis
2.7.1.146 D-glucose substrate inhibition above 200 mM. At 10 mM 20% inhibition of phosphofructokinase activity Methanococcus maripaludis
2.7.1.146 EDTA 50 mM, complete inhibition; complete loss of activity; completely abolishes activity Methanococcus maripaludis
2.7.1.147 ADP strong inhibition effect in the reverse glucokinase reaction Methanococcus maripaludis
2.7.1.147 AMP strong substrate inhibition effect in the reverse glucokinase reaction; substrate inhibition effect (Ki of 0.4 mM at 10 mM of glucose 6-phosphate). At a glucose 6-phosphate concentration of 0.5 mM, a sigmoidal behavior is observed, along with an even more pronounced inhibition Methanococcus maripaludis
2.7.1.147 D-fructose
-
Methanococcus maripaludis
2.7.1.147 D-fructose 6-phosphate competitive inhibition of D-fructose 6-phosphate when glucose is used as the variable substrate; strong inhibition of glucokinase activity, competitive inhibion versus D-glucose as variable substrate Methanococcus maripaludis
2.7.1.147 D-glucose substrate inhibition above 200 mM. At 10 mM 20% inhibition of phosphofructokinase activity Methanococcus maripaludis
2.7.1.147 EDTA 50 mM, complete inhibition; complete loss of activity; completely abolishes activity Methanococcus maripaludis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.146 0.065
-
D-fructose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 0.065
-
D-fructose 6-phosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 0.1 2 D-glucose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 0.1 2 D-fructose 1,6-bisphosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 0.12
-
D-fructose 1,6-bisphosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 8
-
ADP pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 8
-
ADP pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 40
-
D-glucose pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 0.065
-
D-fructose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 0.1 2 D-glucose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 0.62
-
D-glucose 6-phosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 0.623
-
D-glucose 6-phosphate pH 7.8, 30°C Methanococcus maripaludis
2.7.1.147 16
-
ADP pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 16
-
ADP pH 7.8, 30°C Methanococcus maripaludis
2.7.1.147 40
-
D-glucose pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 40
-
D-glucose pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 40
-
D-glucose pH 7.8, 30°C Methanococcus maripaludis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 Co2+ 2 mM, divalent metal ion required, activation of phosphofructokinase activity with Co2+ is about 70% compared to the activation with Mg2+, activation of glucokinase activity is about 35% compared to the activation with Mg2+ Methanococcus maripaludis
2.7.1.146 Co2+ among the divalent metal cations tested, the highest activity is observed in the presence of Mg2+, although, in the presence of Co2+, Ni2+ and Mn2+, significant activity is also measured Methanococcus maripaludis
2.7.1.146 Co2+ 2 mM, about 70% of the activity with Mg2+ Methanococcus maripaludis
2.7.1.146 Mg2+ highest activity in the presence of Mg2+ Methanococcus maripaludis
2.7.1.146 Mg2+ 2 mM, divalent metal ion required, highest activity is observed in the presence of Mg2+ Methanococcus maripaludis
2.7.1.146 Mg2+ among the divalent metal cations tested, the highest activity is observed in the presence of Mg2+, although, in the presence of Co2+, Ni2+ and Mn2+, significant activity is also measured Methanococcus maripaludis
2.7.1.146 Mn2+ 2 mM, divalent metal ion required, activation of phosphofructokinase activity with Co2+ is about 25% compared to the activation with Mg2+, activation of glucokinase activity is about 20% compared to the activation with Mg2+ Methanococcus maripaludis
2.7.1.146 Mn2+ among the divalent metal cations tested, the highest activity is observed in the presence of Mg2+, although, in the presence of Co2+, Ni2+ and Mn2+, significant activity is also measured Methanococcus maripaludis
2.7.1.146 Mn2+ 2 mM, about 25% of the activity with Mg2+ Methanococcus maripaludis
2.7.1.146 additional information divalent cation required, with highest activity in the presence of Mg2+ Methanococcus maripaludis
2.7.1.146 Ni2+ 2 mM, divalent metal ion required, activation of phosphofructokinase activity with Co2+ is about 30% compared to the activation with Mg2+, activation of glucokinase activity is less than 10% compared to the activation with Mg2+ Methanococcus maripaludis
2.7.1.146 Ni2+ among the divalent metal cations tested, the highest activity is observed in the presence of Mg2+, although, in the presence of Co2+, Ni2+ and Mn2+, significant activity is also measured Methanococcus maripaludis
2.7.1.147 Co2+ 2 mM, divalent metal ion required, activation of phosphofructokinase activity with Co2+ is about 70% compared to the activation with Mg2+, activation of glucokinase activity is about 35% compared to the activation with Mg2+ Methanococcus maripaludis
2.7.1.147 Co2+ among the divalent metal cations tested, the highest activity is observed in the presence of Mg2+, although, in the presence of Co2+, Ni2+ and Mn2+, significant activity is also measured Methanococcus maripaludis
2.7.1.147 Co2+ 2 mM, about 40% of the activity with Mg2+ Methanococcus maripaludis
2.7.1.147 Mg2+ highest activity in the presence of Mg2+ Methanococcus maripaludis
2.7.1.147 Mg2+ 2 mM, divalent metal ion required, highest activity is observed in the presence of Mg2+ Methanococcus maripaludis
2.7.1.147 Mg2+ among the divalent metal cations tested, the highest activity is observed in the presence of Mg2+, although, in the presence of Co2+, Ni2+ and Mn2+, significant activity is also measured Methanococcus maripaludis
2.7.1.147 Mn2+ 2 mM, divalent metal ion required, activation of phosphofructokinase activity with Co2+ is about 25% compared to the activation with Mg2+, activation of glucokinase activity is about 20% compared to the activation with Mg2+ Methanococcus maripaludis
2.7.1.147 Mn2+ among the divalent metal cations tested, the highest activity is observed in the presence of Mg2+, although, in the presence of Co2+, Ni2+ and Mn2+, significant activity is also measured Methanococcus maripaludis
2.7.1.147 Mn2+ 2 mM, about 20% of the activity with Mg2+ Methanococcus maripaludis
2.7.1.147 additional information divalent cation required, with highest activity in the presence of Mg2+ Methanococcus maripaludis
2.7.1.147 Ni2+ 2 mM, divalent metal ion required, activation of phosphofructokinase activity with Co2+ is about 30% compared to the activation with Mg2+, activation of glucokinase activity is less than 10% compared to the activation with Mg2+ Methanococcus maripaludis
2.7.1.147 Ni2+ among the divalent metal cations tested, the highest activity is observed in the presence of Mg2+, although, in the presence of Co2+, Ni2+ and Mn2+, significant activity is also measured Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate Methanococcus maripaludis
-
AMP + D-fructose 1,6-bisphosphate
-
ir
2.7.1.146 ADP + D-glucose Methanococcus maripaludis the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate. Binding of both substrates to the same active site. At a sugar concentration of 10 mM the acctivity with D-fructose 6-phosphate is about 75% compared to the activity with D-glucose. No activity in presence of ATP. kcat/KM for the phosphorylation of D-fructose 6-phosphate is 440fold higher than the kcat/Km for the phosphorylation of glucose. Analysis of the kcat/Km ratios shows that the glucose dephosphorylation is 2fold more effective than the phosphorylation AMP + D-glucose 6-phosphate
-
r
2.7.1.147 ADP + D-glucose Methanococcus maripaludis
-
AMP + D-glucose 6-phosphate
-
r
2.7.1.147 ADP + D-glucose Methanococcus maripaludis the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate. Binding of both substrates to the same active site. At a sugar concentration of 10 mM the acctivity with D-fructose 6-phosphate is about 75% compared to the activity with D-glucose. No activity in presence of ATP. kcat/KM for the phosphorylation of D-fructose 6-phosphate is 440fold higher than the kcat/Km for the phosphorylation of glucose. Analysis of the kcat/Km ratios shows that the glucose dephosphorylation is 2fold more effective than the phosphorylation AMP + D-glucose 6-phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Methanococcus maripaludis Q6LXQ3
-
-
2.7.1.146 Methanococcus maripaludis Q6LXQ3 bifunctional ADP-dependent phosphofructokinase/glucokinase, reactions of EC 2.7.1.147 and EC 2.7.1.146, respectively
-
2.7.1.147 Methanococcus maripaludis Q6LXQ3
-
-
2.7.1.147 Methanococcus maripaludis Q6LXQ3 bifunctional ADP-dependent phosphofructokinase/glucokinase, reactions of EC 2.7.1.147 and EC 2.7.1.146, respectively
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.146
-
Methanococcus maripaludis
2.7.1.147
-
Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate
-
Methanococcus maripaludis AMP + D-fructose 1,6-bisphosphate
-
ir
2.7.1.146 ADP + D-fructose 6-phosphate the bifunctional enzyme is able to phosphorylate D-glucose and beta-D-fructose 6-phosphate. The results of molecular modeling show that both sugars are bound to the enzyme by essentially the same residues except for N203, which establishes an interaction only when the substrate is D-fructose 6-phosphate, and E79, which interacts only with glucose. The enzyme shows higher activity with glucose compared to that obtained with beta-D-fructose 6-phosphate. beta-D-Fructose 6-phosphate shows 75% of the activity measured with glucose. In the presence of ATP, no activity is detected. Phosphatase activity is 67-fold lower than the kinase activity Methanococcus maripaludis AMP + D-fructose 1,6-bisphosphate
-
ir
2.7.1.146 ADP + D-fructose 6-phosphate the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate. Binding of both substrates to the same active site. At a sugar concentration of 10 mM the activity with D-fructose 6-phosphate is about 75% compared to the activity with D-glucose. No activity in presence of ATP. kcat/KM for the phosphorylation of D-fructose 6-phosphate is 440fold higher than the kcat/Km for the phosphorylation of glucose Methanococcus maripaludis AMP + D-fructose 1,6-bisphosphate
-
ir
2.7.1.146 ADP + D-fructose 6-phosphate kinetics show hyperbolic behavior Methanococcus maripaludis AMP + D-fructose 1,6-bisphosphate
-
r
2.7.1.146 ADP + D-glucose the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate. Binding of both substrates to the same active site. At a sugar concentration of 10 mM the acctivity with D-fructose 6-phosphate is about 75% compared to the activity with D-glucose. No activity in presence of ATP. kcat/KM for the phosphorylation of D-fructose 6-phosphate is 440fold higher than the kcat/Km for the phosphorylation of glucose. Analysis of the kcat/Km ratios shows that the glucose dephosphorylation is 2fold more effective than the phosphorylation Methanococcus maripaludis AMP + D-glucose 6-phosphate
-
r
2.7.1.146 ADP + D-glucose the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate.Binding of both substrates to the same active site. At a sugar concentration of 10 mM the acctivity with D-fructose 6-phosphate is about 75% compared to the activity with D-glucose. No activity in presence of ATP. kcat/KM for the phosphorylation of D-fructose 6-phosphate is 440fold higher than the kcat/Km for the phosphorylation of glucose. Analysis of the kcat/Km ratios shows that the glucose dephosphorylation is 2fold more effective than the phosphorylation Methanococcus maripaludis AMP + D-glucose 6-phosphate
-
r
2.7.1.146 AMP + D-fructose 1,6-bisphosphate
-
Methanococcus maripaludis ADP + D-fructose 6-phosphate
-
r
2.7.1.146 CDP + beta-D-fructose 6-phosphate about 15% compared to the activity with ADP Methanococcus maripaludis CMP + D-fructose 1,6-bisphosphate
-
ir
2.7.1.146 additional information less than 10% activity compared to the activity with D-glucose and ADP: L-rhamnose, D-arabinose, D-lyxose, D-fucose, D-galactose, D-mannose, D-fructose, 2-deoxyglucose, D-glucosamine, D-xylose, maltose, lactose Methanococcus maripaludis ?
-
?
2.7.1.146 additional information bifunctional ADP-dependent phosphofructokinase/glucokinase, reactions of EC 2.7.1.147 and EC 2.7.1.146, respectively. The rate at which fructose 6-phosphate is phosphorylated is 440fold higher than the glucose phosphorylation rate Methanococcus maripaludis ?
-
?
2.7.1.146 UDP + D-glucose the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate. Activity with UDP and D-glucose is about 20% compared to the activity with ADP and D-glucose Methanococcus maripaludis UMP + D-glucose 6-phosphate
-
r
2.7.1.147 ADP + D-fructose 6-phosphate the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate. Binding of both substrates to the same active site. At a sugar concentration of 10 mM the activity with D-fructose 6-phosphate is about 75% compared to the activity with D-glucose. No activity in presence of ATP. kcat/KM for the phosphorylation of D-fructose 6-phosphate is 440fold higher than the kcat/Km for the phosphorylation of glucose Methanococcus maripaludis AMP + D-fructose 1,6-bisphosphate
-
ir
2.7.1.147 ADP + D-glucose
-
Methanococcus maripaludis AMP + D-glucose 6-phosphate
-
r
2.7.1.147 ADP + D-glucose the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate. Binding of both substrates to the same active site. At a sugar concentration of 10 mM the acctivity with D-fructose 6-phosphate is about 75% compared to the activity with D-glucose. No activity in presence of ATP. kcat/KM for the phosphorylation of D-fructose 6-phosphate is 440fold higher than the kcat/Km for the phosphorylation of glucose. Analysis of the kcat/Km ratios shows that the glucose dephosphorylation is 2fold more effective than the phosphorylation Methanococcus maripaludis AMP + D-glucose 6-phosphate
-
r
2.7.1.147 ADP + D-glucose the bifunctional enzyme is able to phosphorylate D-glucose and beta-D-fructose 6-phosphate. The results of molecular modeling show that both sugars are bound to the enzyme by essentially the same residues except for N203, which establishes an interaction only when the substrate is D-fructose 6-phosphate, and E79, which interacts only with glucose. The enzyme shows higher activity with glucose compared to that obtained with beta-D-fructose 6-phosphate. beta-D-Fructose 6-phosphate shows 75% of the activity measured with glucose. In the presence of ATP, no activity is detected Methanococcus maripaludis AMP + D-glucose 6-phosphate
-
r
2.7.1.147 ADP + D-glucose the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate.Binding of both substrates to the same active site. At a sugar concentration of 10 mM the acctivity with D-fructose 6-phosphate is about 75% compared to the activity with D-glucose. No activity in presence of ATP. kcat/KM for the phosphorylation of D-fructose 6-phosphate is 440fold higher than the kcat/Km for the phosphorylation of glucose. Analysis of the kcat/Km ratios shows that the glucose dephosphorylation is 2fold more effective than the phosphorylation Methanococcus maripaludis AMP + D-glucose 6-phosphate
-
r
2.7.1.147 AMP + D-glucose 6-phosphate
-
Methanococcus maripaludis ADP + D-glucose
-
r
2.7.1.147 GDP + D-glucose about 10% compared to the activity with ADP Methanococcus maripaludis GMP + D-glucose 6-phosphate
-
r
2.7.1.147 additional information less than 10% compared to the activity with D-glucose and ADP: L-rhamnose, D-arabinose, D-lyxose, D-fucose, D-galactose, D-mannose, D-fructose, 2-deoxyglucose, D-glucosamine, D-xylose, maltose, lactose Methanococcus maripaludis ?
-
?
2.7.1.147 additional information bifunctional ADP-dependent phosphofructokinase/glucokinase, reactions of EC 2.7.1.147 and EC 2.7.1.146, respectively. The rate at which fructose 6-phosphate is phosphorylated is 440fold higher than the glucose phosphorylation rate Methanococcus maripaludis ?
-
?
2.7.1.147 TDP + D-glucose about 10% compared to the activity with ADP Methanococcus maripaludis TMP + D-glucose 6-phosphate
-
r
2.7.1.147 UDP + D-glucose about 20% compared to the activity with ADP Methanococcus maripaludis UMP + D-glucose 6-phosphate
-
r
2.7.1.147 UDP + D-glucose the enzyme phosphorylates both D-glucose and D-fructose 6-phosphate. Activity with UDP and D-glucose is about 20% compared to the activity with ADP and D-glucose Methanococcus maripaludis UMP + D-glucose 6-phosphate
-
r
2.7.1.147 UDP + D-glucose about 20% of the activity with ADP Methanococcus maripaludis UMP + D-glucose 6-phosphate
-
r

Synonyms

EC Number Synonyms Comment Organism
2.7.1.146 bifunctional ADP-dependent phosphofructokinase/glucokinase
-
Methanococcus maripaludis
2.7.1.146 MmPFK/GK
-
Methanococcus maripaludis
2.7.1.146 pfkC
-
Methanococcus maripaludis
2.7.1.147 bifunctional ADP-dependent phosphofructokinase/glucokinase
-
Methanococcus maripaludis
2.7.1.147 MmPFK/GK
-
Methanococcus maripaludis
2.7.1.147 pfkC
-
Methanococcus maripaludis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.146 0.45
-
D-fructose 1,6-bisphosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 0.45
-
D-glucose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 0.45
-
D-fructose 1,6-bisphosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 16.5
-
D-fructose 6-phosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 16.5
-
D-fructose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 23
-
D-glucose pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 30
-
ADP pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 30
-
ADP pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 0.45
-
D-glucose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 0.678
-
D-glucose 6-phosphate pH 7.8, 30°C Methanococcus maripaludis
2.7.1.147 0.68
-
D-glucose 6-phosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 16.5
-
D-fructose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 23
-
D-glucose pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 23
-
D-glucose pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 23
-
D-glucose pH 7.8, 30°C Methanococcus maripaludis
2.7.1.147 48
-
ADP pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 48
-
ADP pH 7.8, 30°C Methanococcus maripaludis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.146 6.5
-
-
Methanococcus maripaludis
2.7.1.146 6.5
-
phosphofructokinase activity Methanococcus maripaludis
2.7.1.146 7
-
-
Methanococcus maripaludis
2.7.1.146 7
-
glucokinase activity Methanococcus maripaludis
2.7.1.147 6.5
-
phosphofructokinase activity Methanococcus maripaludis
2.7.1.147 7
-
-
Methanococcus maripaludis
2.7.1.147 7
-
glucokinase activity Methanococcus maripaludis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.146 5 7 pH 5.0: about 50% of maximal activity, pH 7.0: about 65% of maximal activity Methanococcus maripaludis
2.7.1.146 5 7 pH 5.0: about 50% of maximal phosphofructokinase activity, pH 7.0: about 65% of maximal phosphofructokinase activity Methanococcus maripaludis
2.7.1.146 5.5 7.5 pH 5.5: about 50% of maximal phosphofructokinase activity, pH 7.5: about 75% of maximal phosphofructokinase activity Methanococcus maripaludis
2.7.1.147 5 7 pH 5.0: about 50% of maximal phosphofructokinase activity, pH 7.0: about 65% of maximal phosphofructokinase activity Methanococcus maripaludis
2.7.1.147 5.5 7.5 pH 5.5: about 50% of maximal phosphofructokinase activity, pH 7.5: about 75% of maximal phosphofructokinase activity Methanococcus maripaludis
2.7.1.147 5.5 8 pH 5.5: about 50% of maximal activity, pH 8.0: about 30% of maximal activity Methanococcus maripaludis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.146 0.226
-
D-fructose 6-phosphate pH 7.5, 30°C, glucokinase reaction Methanococcus maripaludis
2.7.1.146 0.4
-
AMP pH 7.5, 30°C, reverse glucokinase reaction Methanococcus maripaludis
2.7.1.146 54
-
ADP pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 54
-
ADP pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 895
-
D-glucose pH 7.5, 30°C Methanococcus maripaludis
2.7.1.147 0.226
-
D-fructose 6-phosphate pH 7.8, 30°C Methanococcus maripaludis
2.7.1.147 0.226
-
D-fructose 6-phosphate pH 7.5, 30°C, glucokinase reaction Methanococcus maripaludis
2.7.1.147 0.4
-
AMP pH 7.5, 30°C, reverse glucokinase reaction Methanococcus maripaludis
2.7.1.147 0.4
-
AMP pH 7.8, 30°C. Substrate inhibition effect (Ki of 0.4 mM at 10 mM of glucose 6-phosphate). At a glucose 6-phosphate concentration of 0.5 mM, a sigmoidal behavior is observed, along with an even more pronounced inhibition Methanococcus maripaludis
2.7.1.147 2 5 ADP pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 2 5 ADP pH 7.8, 30°C, glucokinase Methanococcus maripaludis
2.7.1.147 895
-
D-glucose pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 895
-
D-glucose pH 7.5, 30°C Methanococcus maripaludis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.146 0.08
-
pH 7.5, 30°C, reverse glucokinase reaction Methanococcus maripaludis ADP
2.7.1.146 0.22
-
pH 6.5, 30°C Methanococcus maripaludis D-fructose 6-phosphate
2.7.1.146 0.22
-
pH 7.5, 30°C, glucokinase reaction Methanococcus maripaludis D-fructose 6-phosphate
2.7.1.146 40
-
pH 6.5, 30°C Methanococcus maripaludis D-glucose
2.7.1.146 40
-
pH 7.5, 30°C, phosphopfructokinase reaction Methanococcus maripaludis D-fructose
2.7.1.147 0.08
-
pH 7.5, 30°C, reverse glucokinase reaction Methanococcus maripaludis ADP
2.7.1.147 0.22
-
pH 7.5, 30°C, glucokinase reaction Methanococcus maripaludis D-fructose 6-phosphate
2.7.1.147 40
-
pH 7.5, 30°Cphosphopfructokinase reaction Methanococcus maripaludis D-fructose

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.146 0.57
-
D-glucose pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 1.09
-
D-glucose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 3
-
ADP pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 3.75
-
ADP pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 3.75
-
D-fructose 1,6-bisphosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 3.75
-
D-fructose 1,6-bisphosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.146 253
-
D-fructose 6-phosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.146 253
-
D-fructose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 0.57
-
D-glucose pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 0.575
-
D-glucose pH 7.8, 30°C Methanococcus maripaludis
2.7.1.147 0.58
-
D-glucose pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 1.09
-
D-glucose 6-phosphate pH 7.8, 30°C Methanococcus maripaludis
2.7.1.147 1.09
-
D-glucose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis
2.7.1.147 1.1
-
D-glucose 6-phosphate pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 3
-
ADP pH 7.8, 30°C Methanococcus maripaludis
2.7.1.147 3
-
ADP pH 7.0, 30°C Methanococcus maripaludis
2.7.1.147 253
-
D-fructose 6-phosphate pH 6.5, 30°C Methanococcus maripaludis