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Literature summary extracted from

  • Singleton, M.R.; Taylor, S.J.; Parrat, J.S.; Littlechild, J.A.
    Cloning, expression, and characterization of pyrrolidone carboxyl peptidase from the archaeon Thermococcus litoralis (2000), Extremophiles, 4, 297-303.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.3 expressed in Escherichia coli under the control of an inducible tac promoter Thermococcus litoralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.3 iodoacetamide
-
 Thermococcus litoralis
3.4.19.3 N-ethylmaleimide
-
 Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.3 24747
-
 4 * 24747, calculated from sequence Thermococcus litoralis
3.4.19.3 80000 108000 gel filtration Thermococcus litoralis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.19.3 acetonitrile at 50% (v/v) concentrations more than 50% of the initial activity is retained Thermococcus litoralis
3.4.19.3 Ethanol the enzyme retains stability at concentrations up to 90% (v/v) Thermococcus litoralis
3.4.19.3 isopropanol at 50% (v/v) concentrations more than 50% of the initial activity is retained Thermococcus litoralis
3.4.19.3 Methanol the enzyme retains stability at concentrations up to 75% (v/v) Thermococcus litoralis

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.3 Thermococcus litoralis O07883
-
-
3.4.19.3 Thermococcus litoralis DSM 5473 O07883
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.3
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.19.3 3.5
-
 pH 7.5, temperature not specified in the publication Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.3 ethyl L-pyroglutamate + H2O
-
 Thermococcus litoralis L-pyroglutamate + ethanol
-
 ?
3.4.19.3 ethyl L-pyroglutamate + H2O
-
 Thermococcus litoralis DSM 5473 L-pyroglutamate + ethanol
-
 ?
3.4.19.3 L-pyroglutamyl-beta-naphthylamide + H2O
-
 Thermococcus litoralis L-pyroglutamate + 2-naphthylamine
-
 ?
3.4.19.3 L-pyroglutamyl-beta-naphthylamide + H2O
-
 Thermococcus litoralis DSM 5473 L-pyroglutamate + 2-naphthylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.19.3 tetramer 4 * 24747, calculated from sequence Thermococcus litoralis

Synonyms

EC Number Synonyms Comment Organism
3.4.19.3 PCP
-
 Thermococcus litoralis
3.4.19.3 pyrrolidone carboxyl peptidase
-
 Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.19.3 70
-
-
 Thermococcus litoralis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.19.3 50 80 50°C: about 50% of maximal activity Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.3 70
-
 half-life: approximately 1 h Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.3 7.5
-
 assay at Thermococcus litoralis