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Literature summary extracted from

  • Johnsen, U.; Sutter, J.M.; Zaiß, H.; Schönheit, P.
    L-Arabinose degradation pathway in the haloarchaeon Haloferax volcanii involves a novel type of L-arabinose dehydrogenase (2013), Extremophiles, 17, 897-909.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.46 expressed in Escherichia coli Rosetta (DE3)-pLysS cells Haloferax volcanii
1.1.1.376 expression in Escherichia coli Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.46 2
-
 NAD+ at pH 8.5 and 45°C Haloferax volcanii
1.1.1.46 2.1
-
 NADP+ at pH 8.5 and 45°C Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.46 K+ dependent on with with maximal activity at 1.5 M Haloferax volcanii
1.1.1.46 Na+ dependent on with with maximal activity at 1.0 M Haloferax volcanii
1.1.1.376 KCl activity is dependent on KCl and NaCl. Maximal activities are obtained at 1.5 M KCl and 1 M NaCl Haloferax volcanii
1.1.1.376 NaCl activity is dependent on KCl and NaCl. Maximal activities are obtained at 1.5 M KCl and 1 M NaCl Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.46 27800
-
-
 Haloferax volcanii
1.1.1.46 28000
-
 2 * 28000, SDS-PAGE Haloferax volcanii
1.1.1.46 130000
-
 gel filtration Haloferax volcanii
1.1.1.376 28000
-
 4 * 28000, SDS-PAGE Haloferax volcanii
1.1.1.376 130000
-
 gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.46 L-arabinose + NAD+ Haloferax volcanii
-
 L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.46 L-arabinose + NAD+ Haloferax volcanii H26
-
 L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.46 L-arabinose + NAD+ Haloferax volcanii DSM 3757
-
 L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.46 L-arabinose + NADP+ Haloferax volcanii
-
 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.46 L-arabinose + NADP+ Haloferax volcanii H26
-
 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.46 L-arabinose + NADP+ Haloferax volcanii DSM 3757
-
 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.376 L-arabinose + NAD(P)+ Haloferax volcanii the enzyme initiates L-arabinose degradation L-arabinono-1,4-lactone + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.46 Haloferax volcanii D4GP33
-
-
1.1.1.46 Haloferax volcanii H26 D4GP33
-
-
1.1.1.376 Haloferax volcanii D4GP33
-
-
1.1.1.376 Haloferax volcanii DSM 3757 D4GP33
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.46 Sepharose CL 4B column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Haloferax volcanii
1.1.1.376
-
 Haloferax volcanii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.46 refolding buffer contains 3 M KCl, L-arabinose, NADP+ and glutathione Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.46 L-arabinose + NAD+
-
 Haloferax volcanii L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.46 L-arabinose + NAD+
-
 Haloferax volcanii H26 L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.46 L-arabinose + NAD+
-
 Haloferax volcanii DSM 3757 L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.46 L-arabinose + NADP+
-
 Haloferax volcanii L-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.46 L-arabinose + NADP+
-
 Haloferax volcanii H26 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.46 L-arabinose + NADP+
-
 Haloferax volcanii DSM 3757 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.46 additional information no activity with ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose Haloferax volcanii ?
-
 ?
1.1.1.46 additional information no activity with ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose Haloferax volcanii H26 ?
-
 ?
1.1.1.46 additional information no activity with ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose Haloferax volcanii DSM 3757 ?
-
 ?
1.1.1.376 L-arabinose + NAD(P)+ the enzyme initiates L-arabinose degradation Haloferax volcanii L-arabinono-1,4-lactone + NAD(P)H + H+
-
 ?
1.1.1.376 L-arabinose + NAD+ the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+. The enzyme is highly specific for L-arabinose as substrate. D-Ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose are not used at significant rates, measured with both NADP+ and NAD+, respectively, as electron acceptors Haloferax volcanii L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.376 L-arabinose + NAD+ the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+. The enzyme is highly specific for L-arabinose as substrate. D-Ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose are not used at significant rates, measured with both NADP+ and NAD+, respectively, as electron acceptors Haloferax volcanii H26 L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.376 L-arabinose + NAD+ the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+. The enzyme is highly specific for L-arabinose as substrate. D-Ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose are not used at significant rates, measured with both NADP+ and NAD+, respectively, as electron acceptors Haloferax volcanii DSM 3757 L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.376 L-arabinose + NADP+ the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+. The enzyme is highly specific for L-arabinose as substrate. D-Ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose are not used at significant rates, measured with both NADP+ and NAD+, respectively, as electron acceptors Haloferax volcanii L-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.376 L-arabinose + NADP+ the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+. The enzyme is highly specific for L-arabinose as substrate. D-Ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose are not used at significant rates, measured with both NADP+ and NAD+, respectively, as electron acceptors Haloferax volcanii H26 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
1.1.1.376 L-arabinose + NADP+ the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+. The enzyme is highly specific for L-arabinose as substrate. D-Ribose, D-glucose, D-talose, D-galactose, D-arabinose, D-xylose, D-mannose, L-mannose and D-fructose are not used at significant rates, measured with both NADP+ and NAD+, respectively, as electron acceptors Haloferax volcanii DSM 3757 L-arabinono-1,4-lactone + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.46 homotetramer 2 * 28000, SDS-PAGE Haloferax volcanii
1.1.1.46 homotetramer 2 * 27800, calculated from amino acid sequence Haloferax volcanii
1.1.1.376 homotetramer 4 * 28000, SDS-PAGE Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.46 L-arabinose dehydrogenase
-
 Haloferax volcanii
1.1.1.46 L-AraDH
-
 Haloferax volcanii
1.1.1.376 HVO_B0032 locus name Haloferax volcanii
1.1.1.376 L-AraDH
-
 Haloferax volcanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.46 45
-
-
 Haloferax volcanii
1.1.1.376 45
-
-
 Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.46 8.5
-
-
 Haloferax volcanii
1.1.1.376 8.5
-
-
 Haloferax volcanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.46 6 10 50% activity at pH 6.0 and 10.0 Haloferax volcanii
1.1.1.376 6 10 50% of maximal activity is found at pH values of 6 and 10 Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.46 NAD+ the enzyme shows slight preference for NADP+ over NAD+ Haloferax volcanii
1.1.1.46 NADP+ the enzyme shows slight preference for NADP+ over NAD+ Haloferax volcanii
1.1.1.376 NAD+ the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+ Haloferax volcanii
1.1.1.376 NADP+ the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+ Haloferax volcanii

Expression

EC Number Organism Comment Expression
1.1.1.376 Haloferax volcanii transcriptionally induced by both L-arabinose and D-xylose up

General Information

EC Number General Information Comment Organism
1.1.1.46 metabolism the enzyme is functionally involved in L-arabinose catabolism but not in D-xylose degradation Haloferax volcanii
1.1.1.376 malfunction the L-AraDH deletion mutant does not grow on L-arabinose, whereas growth on D-xylose and glucose is unaffected Haloferax volcanii
1.1.1.376 physiological function the enzyme is functionally involved in L-arabinose catabolism but not in D-xylose degradation Haloferax volcanii