EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.337 | del I81/delK82 | phenylpyruvate is the only substrate which is converted at a significant catalytic rate by the mutant enzyme. In the publication this mutant is referred to as Ile100ADELTA/Lys100BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region | Weissella confusa |
1.1.1.337 | del I81/delK82/delN87/delP88 | specific modifications in catalytic rates and substrate recognition | Weissella confusa |
1.1.1.337 | del L 83 | deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Leu101DELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region | Weissella confusa |
1.1.1.337 | delI81 | drastic reductions in the catalytic activity for all tested substrates. In the publication this mutant is referred to as Ile100ADELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region | Weissella confusa |
1.1.1.337 | delK82 | drastic reductions in the catalytic activity for all tested substrates. In the publication this mutant is referred to as Lys100BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region | Weissella confusa |
1.1.1.337 | delN87 | deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Asn105ADELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region | Weissella confusa |
1.1.1.337 | delN87/delP88 | for the deletion mutant enzyme 2-oxo carboxylic acids branched at C4 are better substrates than 2-oxocaproate, the substrate with the best kcat,/KM ratio known for the wild-type enzyme.The mutation results in a 5.2fold increased catalytic efficiency towards 4-methyl-2-oxopentanoate compared to the wild-type enzyme. In the publication this mutant is referred to as Asn105ADELTA/Pro105BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region | Weissella confusa |
1.1.1.337 | delP88 | deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Pro105BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region | Weissella confusa |
1.1.1.337 | additional information | the coenzyme loop, a functional element which is essential for catalysis and substrate specificity, is modified in order to identify the residues involved in the catalytic reaction and substrate specificity | Weissella confusa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.337 | 0.026 | - |
phenylpyruvate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 0.067 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 0.071 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 0.1 | 1 | 2-oxocaproate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 0.1 | 2 | phenylpyruvate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 0.17 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 0.33 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 0.41 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 0.62 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 0.9 | 1 | 4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 1 | 4 | 4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 2 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 2 | 5 | pyruvate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 2.8 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 2.8 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del L83 | Weissella confusa | |
1.1.1.337 | 2.8 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 3.3 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 3.5 | - |
pyruvate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 5 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 5.8 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 6.4 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del L83 | Weissella confusa | |
1.1.1.337 | 9.3 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 11 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del L83 | Weissella confusa | |
1.1.1.337 | 18 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 18 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 19 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 19 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 27 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 30 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 43 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 50 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 62 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 110 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 140 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 200 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.337 | Weissella confusa | P14295 | i.e. Lactobacillus confusus | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.337 | - |
Weissella confusa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.337 | 2-oxocaproate + NADH + H+ | - |
Weissella confusa | 2-hydroxycaproate + NAD+ | - |
r | |
1.1.1.337 | 4-methyl-2-oxopentanoate + NADH + H+ | - |
Weissella confusa | (S)-2-hydroxy-4-methylpentanoate + NAD+ | - |
r | |
1.1.1.337 | phenylpyruvate + NADH + H+ | - |
Weissella confusa | 3-phenyllactate + NAD+ | - |
r | |
1.1.1.337 | pyruvate + NADH + H+ | - |
Weissella confusa | L-lactate + NAD+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.337 | L-2-hydroxyisocaproate dehydrogenase | - |
Weissella confusa |
1.1.1.337 | L-HicDH | - |
Weissella confusa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.337 | 30 | - |
assay at | Weissella confusa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.337 | 0.004 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 0.03 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 0.23 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 0.63 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 0.76 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 1.7 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 8 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 13 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 15 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 17 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 20 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 25 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 39 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 40 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 46 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 90 | - |
pyruvate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 280 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 490 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 620 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 750 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 920 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 1000 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del L 83 | Weissella confusa | |
1.1.1.337 | 1200 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del L 83 | Weissella confusa | |
1.1.1.337 | 2400 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 2600 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 4200 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del L 83 | Weissella confusa | |
1.1.1.337 | 4400 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 7300 | - |
phenylpyruvate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 12000 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 15000 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 19000 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 21000 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 89000 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 360000 | - |
2-oxocaproate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 520000 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.337 | 7 | - |
assay at | Weissella confusa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.337 | 0.00013 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 0.00021 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 0.0038 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 0.025 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 0.035 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 0.092 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 0.32 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 0.6 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 0.63 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 0.68 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 0.91 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 0.92 | - |
pyruvate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 1.4 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81 | Weissella confusa | |
1.1.1.337 | 1.8 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 8 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 8.4 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 15 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delK82 | Weissella confusa | |
1.1.1.337 | 26 | - |
pyruvate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 160 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme del L 83 | Weissella confusa | |
1.1.1.337 | 380 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme del L 83 | Weissella confusa | |
1.1.1.337 | 790 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82 | Weissella confusa | |
1.1.1.337 | 2200 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 2900 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 4300 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 | Weissella confusa | |
1.1.1.337 | 4300 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme del L 83 | Weissella confusa | |
1.1.1.337 | 6300 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 8700 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delP88 | Weissella confusa | |
1.1.1.337 | 16000 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 34000 | - |
2-oxocaproate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 36000 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 46000 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delN87 | Weissella confusa | |
1.1.1.337 | 190000 | - |
4-methyl-2-oxopentanoate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 270000 | - |
phenylpyruvate | pH 7.0, 30°C, mutant enzyme delN87/delP88 | Weissella confusa | |
1.1.1.337 | 280000 | - |
phenylpyruvate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa | |
1.1.1.337 | 3300000 | - |
2-oxocaproate | pH 7.0, 30°C, wild-type enzyme | Weissella confusa |