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Literature summary extracted from

  • Jiang, M.; Chen, X.; Liang, L.; Liu, R.; Wan, Q.; Wu, M.; Zhang, H.; Ma, J.; Chen, K.; Ouyang, P.
    Co-expression of phosphoenolpyruvate carboxykinase and nicotinic acid phosphoribosyltransferase for succinate production in engineered Escherichia coli (2013), Enzyme Microb. Technol., 56, 8-14.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.49 expressed in Escherichia coli BA203 cells Bacillus subtilis
6.3.4.21 expressed in Escherichia coli strains BA203, BA204, BA206, and BA209 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.49 ATP + phosphoenolpyruvate + CO2 Bacillus subtilis
-
 ADP + oxaloacetate
-
 ?
4.1.1.49 ATP + phosphoenolpyruvate + CO2 Bacillus subtilis 168
-
 ADP + oxaloacetate
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Escherichia coli
-
 beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.49 Bacillus subtilis
-
-
-
4.1.1.49 Bacillus subtilis 168
-
-
-
6.3.4.21 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.21 0.08
-
 recombinant enzyme from Escherichia coli strain BA203, pH and temperature not specified in the publication Escherichia coli
6.3.4.21 0.74
-
 recombinant enzyme from Escherichia coli strain BA204, pH and temperature not specified in the publication Escherichia coli
6.3.4.21 17.31
-
 recombinant enzyme from Escherichia coli strain BA206, pH and temperature not specified in the publication Escherichia coli
6.3.4.21 23.72
-
 recombinant enzyme from Escherichia coli strain BA207, pH and temperature not specified in the publication Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.49 ATP + phosphoenolpyruvate + CO2
-
 Bacillus subtilis ADP + oxaloacetate
-
 ?
4.1.1.49 ATP + phosphoenolpyruvate + CO2
-
 Bacillus subtilis 168 ADP + oxaloacetate
-
 ?
6.3.4.21 nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
 Escherichia coli beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.49 ATP-forming PEP-carboxykinase
-
 Bacillus subtilis
4.1.1.49 PEPCK
-
 Bacillus subtilis
4.1.1.49 PPC
-
 Bacillus subtilis
6.3.4.21 NAPRTase
-
 Escherichia coli
6.3.4.21 nicotinic acid phosphoribosyltransferase
-
 Escherichia coli
6.3.4.21 pncB
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.49 ATP
-
 Bacillus subtilis
6.3.4.21 ATP
-
 Escherichia coli