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Literature summary extracted from

  • Slutskaya, E.S.; Bezsudnova, E.Y.; Mardanov, A.V.; Gumerov, V.M.; Rakitina, T.V.; Popov, V.O.; Lipkin, V.M.
    Characterization of a novel M42 aminopeptidase from crenarchaeon Desulfurococcus kamchatkensis (2012), Dokl. Biochem. Biophys., 442, 30-32.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 bestatin
-
 Desulfurococcus amylolyticus
3.4.11.1 EDTA
-
 Desulfurococcus amylolyticus
3.4.11.1 additional information leupeptin, aprotinin, and pepstatin virtually have no effect on the enzyme activity Desulfurococcus amylolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 1.2
-
 L-Leu-4-nitroanilide pH 7.5, 50°C, enzyme activated by heating at 70°C Desulfurococcus amylolyticus
3.4.11.1 1.8
-
 L-Leu-4-nitroanilide pH 7.5, 50°C Desulfurococcus amylolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mg2+ maximum activity of the apoenzyme is observed in the presence of Mg2+, Mn2+, and Zn2+ Desulfurococcus amylolyticus
3.4.11.1 Mn2+ maximum activity of the apoenzyme is observed in the presence of Mg2+, Mn2+, and Zn2+ Desulfurococcus amylolyticus
3.4.11.1 Zn2+ maximum activity of the apoenzyme is observed in the presence of Mg2+, Mn2+, and Zn2+ Desulfurococcus amylolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 43200
-
 2 * 43200, octamer/dimer mixture at the ratio 2:1, SDS-PAGE Desulfurococcus amylolyticus
3.4.11.1 43200
-
 8 * 43200, octamer/dimer mixture at the ratio 2:1, SDS-PAGE Desulfurococcus amylolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Desulfurococcus amylolyticus B8D484
-
-
3.4.11.1 Desulfurococcus amylolyticus DSM 18924 B8D484
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1
-
 Desulfurococcus amylolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 4.1
-
 pH 7.5, 50°C, substrate: L-Leu-4-nitroanilide Desulfurococcus amylolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Desulfurococcus amylolyticus L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Desulfurococcus amylolyticus DSM 18924 L-Leu + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 dimer 2 * 43200, octamer/dimer mixture at the ratio 2:1, SDS-PAGE Desulfurococcus amylolyticus
3.4.11.1 octamer 8 * 43200, octamer/dimer mixture at the ratio 2:1, SDS-PAGE Desulfurococcus amylolyticus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 APDkam589
-
 Desulfurococcus amylolyticus
3.4.11.1 M42 aminopeptidase
-
 Desulfurococcus amylolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 50
-
 assay at Desulfurococcus amylolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.1 additional information
-
 heating APDkam589 (600 µg/ml) at 70°C for 1 h lead to an increase in the specific activity to 20-22 U/mg. Further heating of the enzyme preparation does not lead to an increase of the activity. After cooling to room temperature the resulting enzyme activity is retained within 12 h. Apparently, the heating of the protein switches it to a more active conformation Desulfurococcus amylolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 5.4
-
 L-Leu-4-nitroanilide pH 7.5, 50°C Desulfurococcus amylolyticus
3.4.11.1 19
-
 L-Leu-4-nitroanilide pH 7.5, 50°C, enzyme activated by heating at 70°C Desulfurococcus amylolyticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.1 3
-
 L-Leu-4-nitroanilide pH 7.5, 50°C Desulfurococcus amylolyticus
3.4.11.1 15.8
-
 L-Leu-4-nitroanilide pH 7.5, 50°C, enzyme activated by heating at 70°C Desulfurococcus amylolyticus