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Literature summary extracted from
- Targeting bacterial membranes: identification of Pseudomonas aeruginosa D-arabinose-5P isomerase and NMR characterisation of its substrate recognition and binding properties (2011), ChemBioChem, 12, 719-727.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.13 | expressed in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.1.13 | H190A | inactive | Pseudomonas aeruginosa |
| 5.3.1.13 | H85A | inactive | Pseudomonas aeruginosa |
| 5.3.1.13 | K56A | the mutant shows 0.31% residual activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.13 | 34000 | - |
x * 34000, SDS-PAGE | Pseudomonas aeruginosa |
| 5.3.1.13 | 34067 | - |
x * 34067, calculated from amino acid sequence | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.13 | D-Arabinose 5-phosphate | Pseudomonas aeruginosa | - |
D-Ribulose 5-phosphate | - |
r |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.13 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.13 | Ni-NTA agarose column chromatography | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.13 | 0.092 | - |
mutant enzyme K56A, in Tris·HCl, (pH 8.5, 50 mM), at 37°C | Pseudomonas aeruginosa |
| 5.3.1.13 | 29.5 | - |
recombinant wild type enzyme, in Tris·HCl, (pH 8.5, 50 mM), at 37°C | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.13 | 3-deoxy-D-threo-pentofuranose 5-phosphate | the catalytic efficiency is about 1000times lower than for the natural substrate D-arabinose 5-phosphate | Pseudomonas aeruginosa | 3-deoxy-D-glycero-pentulose 5-phosphate | - |
? | |
| 5.3.1.13 | 4-deoxy-D-threo-pentofuranose 5-phosphate | the catalytic efficiency is comparable with the natural substrate D-arabinose 5-phosphate | Pseudomonas aeruginosa | 4-deoxy-D-glycero-pentulose 5-phosphate | - |
? | |
| 5.3.1.13 | D-Arabinose 5-phosphate | - |
Pseudomonas aeruginosa | D-Ribulose 5-phosphate | - |
r | |
| 5.3.1.13 | additional information | no activity with D-ribose 5-phosphate | Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.13 | ? | x * 34000, SDS-PAGE | Pseudomonas aeruginosa |
| 5.3.1.13 | ? | x * 34067, calculated from amino acid sequence | Pseudomonas aeruginosa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.13 | API | - |
Pseudomonas aeruginosa |
| 5.3.1.13 | D-arabinose-5P isomerase | - |
Pseudomonas aeruginosa |
| 5.3.1.13 | KdsD | - |
Pseudomonas aeruginosa |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.3.1.13 | metabolism | the enzyme is involved in the biosynthesis of 3-deoxy-D-manno-oct-2-ulosonic acid and thus of lipopolysaccharide | Pseudomonas aeruginosa |
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